Stacking transgenes in forest trees

C Halpin, W Boerjan - Trends in Plant Science, 2003 - cell.com
Huge potential exists for improving plant raw materials and foodstuffs via metabolic
engineering. To date, progress has mostly been limited to modulating the expression of …

Repression of lignin biosynthesis promotes cellulose accumulation and growth in transgenic trees

WJ Hu, SA Harding, J Lung, JL Popko, J Ralph… - Nature …, 1999 - nature.com
Because lignin limits the use of wood for fiber, chemical, and energy production, strategies
for its downregulation are of considerable interest. We have produced transgenic aspen …

Combinatorial modification of multiple lignin traits in trees through multigene cotransformation

L Li, Y Zhou, X Cheng, J Sun… - Proceedings of the …, 2003 - National Acad Sciences
Lignin quantity and reactivity [which is associated with its syringyl/guaiacyl (S/G) constituent
ratio] are two major barriers to wood-pulp production. To verify our contention that these …

Enabling technologies for manipulating multiple genes on complex pathways

C Halpin, A Barakate, BM Askari, JC Abbott, MD Ryan - Plant Cell Walls, 2001 - Springer
Many complex biochemical pathways in plants have now been manipulated genetically,
usually by suppression or over-expression of single genes. Further exploitation of the …

Control of lignin biosynthesis

JH Christensen, M Baucher, A O'connell… - Molecular Biology of …, 2000 - Springer
The ever increasing worldwide use of forest tree products, which coincides with the
diminishing of natural forests, necessitates programs for efficient tree farming. For this …

Antisense Down-Regulation of 4CL Expression Alters Lignification, Tree Growth, and Saccharification Potential of Field-Grown Poplar

SL Voelker, B Lachenbruch, FC Meinzer… - Plant …, 2010 - academic.oup.com
Transgenic down-regulation of the Pt4CL1 gene family encoding 4-coumarate: coenzyme A
ligase (4CL) has been reported as a means for reducing lignin content in cell walls and …

A gene-editing/complementation strategy for tissue-specific lignin reduction while preserving biomass yield

H Yu, C Liu, RA Dixon - Biotechnology for Biofuels, 2021 - Springer
Background Lignification of secondary cell walls is a major factor conferring recalcitrance of
lignocellulosic biomass to deconstruction for fuels and chemicals. Genetic modification can …

Monolignol biosynthesis and genetic engineering of lignin in trees, a review

VL Chiang - Environmental Chemistry Letters, 2006 - Springer
This paper summarizes our previous and current research on genetic engineering of lignin
biosynthesis for the purposes of improving wood pulping and bleaching efficiency. For these …

Investigating and manipulating lignin biosynthesis in the postgenomic era

C Halpin - Advances in botanical research, 2004 - Elsevier
Enormous progress has been made over the last decade in understanding and
manipulating the pathway for lignin biosynthesis in plants. The roles of most of the genes on …

Field trials with lignin-modified transgenic trees

G Pilate, A Dejardin, JC Leple - Advances in Botanical Research, 2012 - Elsevier
For the last 20 years, as a complement to biochemical approaches, lignin-modified
transgenic trees have proven to be invaluable tools for deciphering the lignin biosynthetic …