Nanopore Sequencing Significantly Improves Genome Assembly of the Protozoan Parasite Trypanosoma cruzi

F Díaz-Viraqué, S Pita, G Greif… - Genome biology and …, 2019 - academic.oup.com
Chagas disease was described by Carlos Chagas, who first identified the parasite
Trypanosoma cruzi from a 2-year-old girl called Berenice. Many T. cruzi sequencing projects …

Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome

JL Reis-Cunha, DC Bartholomeu - T. cruzi Infection: Methods and …, 2019 - Springer
Trypanosoma cruzi present one of the most complex parasite genomes sequenced to date.
Among its features are 600-kb-long repetitive multigene families' clusters, hybrid strains, and …

Assembly of highly repetitive genomes using short reads: the genome of discrete typing unit III Trypanosoma cruzi strain 231

RP Baptista, JL Reis-Cunha, JD DeBarry… - Microbial …, 2018 - microbiologyresearch.org
Next-generation sequencing (NGS) methods are low-cost high-throughput technologies that
produce thousands to millions of sequence reads. Despite the high number of raw sequence …

[HTML][HTML] Genomic assemblies of newly sequenced Trypanosoma cruzi strains reveal new genomic expansion and greater complexity

F Callejas-Hernández, A Rastrojo, C Poveda… - Scientific reports, 2018 - nature.com
Chagas disease is a complex illness caused by the protozoan Trypanosoma cruzi
displaying highly diverse clinical outcomes. In this sense, the genome sequence elucidation …

[HTML][HTML] Expanding an expanded genome: long-read sequencing of Trypanosoma cruzi

L Berná, M Rodriguez, ML Chiribao… - Microbial …, 2018 - microbiologyresearch.org
Although the genome of Trypanosoma cruzi, the causative agent of Chagas disease, was
first made available in 2005, with additional strains reported later, the intrinsic genome …

[HTML][HTML] Trypanosoma cruzi Genome 15 Years Later: What Has Been Accomplished?

JL Ramirez - Tropical Medicine and Infectious Disease, 2020 - mdpi.com
On 15 July 2020 was the 15th anniversary of the Science Magazine issue that reported three
trypanosomatid genomes, namely Leishmania major, Trypanosoma brucei, and …

[HTML][HTML] Repeat-Driven Generation of Antigenic Diversity in a Major Human Pathogen, Trypanosoma cruzi

C Talavera-López, LA Messenger, MD Lewis… - Frontiers in cellular …, 2021 - frontiersin.org
Trypanosoma cruzi, a zoonotic kinetoplastid protozoan parasite, is the causative agent of
American trypanosomiasis (Chagas disease). Having a very plastic, repetitive and complex …

[HTML][HTML] Shotgun Sequencing Analysis of Trypanosoma cruzi I Sylvio X10/1 and Comparison with T. cruzi VI CL Brener

O Franzen, S Ochaya, E Sherwood… - PLoS neglected …, 2011 - journals.plos.org
Trypanosoma cruzi is the causative agent of Chagas disease, which affects more than 9
million people in Latin America. We have generated a draft genome sequence of the TcI …

Trypanosoma cruzi diversity in naturally infected nonhuman primates in Louisiana assessed by deep sequencing of the mini-exon gene

C Herrera, A Majeau, P Didier… - Transactions of the …, 2019 - academic.oup.com
Background Trypanosoma cruzi is a zoonotic pathogen of increasing relevance in the USA,
with a growing number of autochthonous cases identified in recent years. The identification …

[HTML][HTML] Deep sequencing reveals multiclonality and new discrete typing units of Trypanosoma cruzi in rodents from the southern United States

H Pronovost, AC Peterson, BG Chavez, MJ Blum… - Journal of Microbiology …, 2020 - Elsevier
Background/purpose The parasitic protozoa Trypanosoma cruzi, is widely distributed
throughout the Americas. We explored the nature of T. cruzi infection in small rodents from …