Rigorous investigation of many functions encoded by cytomegaloviruses (CMVs) requires
analysis in the context of virus-host interactions. To facilitate the construction of rhesus CMV …

Cytomegaloviruses (CMVs) are highly adapted to their host species resulting in strict
species specificity. Hence, in vivo examination of all aspects of CMV biology employs animal …

Clinical human cytomegalovirus (HCMV) strains invariably mutate when propagated in vitro.
Mutations in gene RL13 are selected in all cell types, whereas in fibroblasts mutants in the …

The understanding of human cytomegalovirus (HCMV) biology was long hindered by the
inability to perform efficient viral genetic analysis. This hurdle was recently overcome when …

We have recently introduced a novel procedure for the construction of herpesvirus mutants
that is based on the cloning and mutagenesis of herpesvirus genomes as infectious …

Since cytomegalovirus (CMV) infection is highly species-specific, it is necessary to study
animal cytomegaloviruses to assess viral factors which contribute to pathogenesis. The …

The full-length genome of human cytomegalovirus strain AD169 was cloned as an infectious
bacterial artificial chromosome (BAC) plasmid, pAD/Cre. The BAC vector, flanked by LoxP …

Transcription of the major immediate-early (MIE) genes of cytomegaloviruses (CMV) is
driven by a strong promoter-enhancer (MIEPE) complex. Transactivator proteins encoded by …

For immunological research on the human cytomegalovirus (HCMV), a virus that combines
the broad cell tropism of clinical isolates, efficient replication in cell culture, the complete set …

Systematic mutagenesis of large viral genomes such as those of the cytomegaloviruses
requires strategies for identifying relevant functions as well as for detailed analysis of …