Ligating a protein at a specific site with a tag molecule containing a paramagnetic metal ion
provides a versatile way of generating pseudocontact shifts (PCSs) in nuclear magnetic …

The anisotropic component of the magnetic susceptibility tensor (Δχ tensor) associated with
various paramagnetic metal ions can induce pseudocontact shifts (PCSs) and residual …

Paramagnetic metal ions bound to proteins generate a dipolar field that can be accurately
probed by pseudocontact shifts (PCS) displayed by the protein's nuclear spins. PCS are …

The binding of paramagnetic metal ions to proteins produces a number of different effects on
the NMR spectra of the system. In particular, when the magnetic susceptibility of the metal …

Pseudo contact shifts (PCSs) induced by paramagnetic lanthanide ions fixed in a protein
frame provide long-range distance and angular information, and are valuable for the …

Pseudocontact shifts (PCS) encode long-range information on 3D structures of protein
backbones and side-chains. The level of structural detail that can be obtained increases with …

Pseudocontact shifts (PCSs) generated by paramagnetic lanthanide ions provide valuable
long-range structural information in nuclear magnetic resonance (NMR) spectroscopic …

Highlights•Anisotropic paramagnetic effects include PseudoContact Shifts and RDC.•PCS
provide long-range restraints for structure determination.•Practical aspects of PCS structure …

Pseudocontact shift (PCS) effects induced by a paramagnetic lanthanide bound to a protein
have become increasingly popular in NMR spectroscopy as they yield a complementary set …

Paramagnetic metal ions generate pseudocontact shifts (PCSs) in nuclear magnetic
resonance spectra that are manifested as easily measurable changes in chemical shifts …