Positioning and the specific sequence of each 13‐mer motif are critical for activity of the plasmid RK2 replication origin
L Kowalczyk, M Rajewska… - Molecular microbiology, 2005 - Wiley Online Library
The minimal replication origin of the broad‐host‐range plasmid RK2, oriV, contains five
iterons which are binding sites for the plasmid‐encoded replication initiation protein TrfA …
iterons which are binding sites for the plasmid‐encoded replication initiation protein TrfA …
Replication origin of the broad host range plasmid RK2: positioning of various motifs is critical for initiation of replication
KS Doran, I Konieczny, DR Helinski - Journal of Biological Chemistry, 1998 - ASBMB
The 393-base pair minimal origin, oriV, of plasmid RK2 contains three iterated motifs
essential for initiation of replication: consensus sequences for binding the bacterial DnaA …
essential for initiation of replication: consensus sequences for binding the bacterial DnaA …
Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.
A Blasina, BL Kittell, AE Toukdarian… - Proceedings of the …, 1996 - National Acad Sciences
The broad host range plasmid RK2 replicates and regulates its copy number in a wide range
of Gram-negative bacteria. The plasmid-encoded trans-acting replication protein TrfA and …
of Gram-negative bacteria. The plasmid-encoded trans-acting replication protein TrfA and …
Analysis of mutations in trfA, the replication initiation gene of the broad-host-range plasmid RK2
J Lin, DR Helinski - Journal of bacteriology, 1992 - Am Soc Microbiol
Plasmids with mutations in trfA, the gene encoding the replication initiation protein of the
broad-host-range plasmid RK2, were isolated and characterized. Mutants identified from a …
broad-host-range plasmid RK2, were isolated and characterized. Mutants identified from a …
[HTML][HTML] DNA sequence requirements for interaction of the RK2 replication initiation protein with plasmid origin repeats.
S Perri, DR Helinski - Journal of Biological Chemistry, 1993 - Elsevier
Replication of plasmid RK2 in a variety of Gram-negative bacteria requires its origin of
replication and the plasmid-encoded TrfA proteins (TrfA-33 and TrfA-44). The initiation of …
replication and the plasmid-encoded TrfA proteins (TrfA-33 and TrfA-44). The initiation of …
[HTML][HTML] Essentiality of the three carboxyl-terminal amino acids of the plasmid RK2 replication initiation protein TrfA for DNA binding and replication activity in gram …
JL Cereghino, DR Helinski - Journal of Biological Chemistry, 1993 - Elsevier
In a previous study of mutations in trfA, the gene encoding the replication initiation protein of
the broad host-range plasmid RK2, a carboxyl-terminal deletion of 3 amino acids of the TrfA …
the broad host-range plasmid RK2, a carboxyl-terminal deletion of 3 amino acids of the TrfA …
Dissection of the core and auxiliary sequences in the vegetative replication origin of promiscuous plasmid RK2
DS Shah, MA Cross, D Porter, CM Thomas - Journal of molecular biology, 1995 - Elsevier
The vegetative replication origin (oriV) of promiscuous IncP plasmid RK2 can function in
many Gram-negative bacterial species when supplied with the plasmid-encoded replication …
many Gram-negative bacterial species when supplied with the plasmid-encoded replication …
Host‐dependent requirement for specific DnaA boxes for plasmid RK2 replication
KS Doran, DR Helinski, I Konieczny - Molecular microbiology, 1999 - Wiley Online Library
The replication origin of the broad‐host‐range plasmid RK2, oriV, contains four DnaA boxes,
which bind the DnaA protein isolated from Escherichia coli. Using a transformation assay …
which bind the DnaA protein isolated from Escherichia coli. Using a transformation assay …
Role of TrfA and DnaA proteins in origin opening during initiation of DNA replication of the broad host range plasmid RK2
I Konieczny, KS Doran, DR Helinski… - Journal of Biological …, 1997 - ASBMB
The Escherichia coli protein DnaA and the plasmid RK2-encoded TrfA protein are required
for initiation of replication of the broad host range plasmid RK2. The TrfA protein has been …
for initiation of replication of the broad host range plasmid RK2. The TrfA protein has been …
The sequence encoding the 43-kilodalton trfA protein is required for efficient replication or maintenance of minimal RK2 replicons in Pseudomonas aeruginosa
RH Durland, DR Helinski - Plasmid, 1987 - Elsevier
The trfA gene of the broad-host-range plasmid RK2 encodes two proteins of 43-and 32-kDa
by initiating translation at either of two in-phase AUG codons in a single open reading frame …
by initiating translation at either of two in-phase AUG codons in a single open reading frame …