Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable
measure for taxonomic profiling of bacterial communities. This chapter describes the …

Since its introduction, nanopore sequencing has enhanced our ability to study complex
microbial samples through the possibility to sequence long reads in real time using …

Respiratory tract infection (RTI) remains a significant cause of morbidity and mortality across
the globe. The optimal management of RTI relies upon timely pathogen identification via …

Cancer patients are at high risk of infections and infection-related mortality; thereby, prompt
diagnosis and precise anti-infectives treatment are critical. This study aimed to evaluate the …

Amplicon sequencing of the 16S rRNA gene is commonly used for the identification of
bacterial isolates in diagnostic laboratories and mostly relies on the Sanger sequencing …

Urinary tract infections (UTIs) are among the most common acquired bacterial infections in
humans. The current gold standard method for identification of uropathogens in clinical …

Fast and accurate identification of pathogens is an essential task in healthcare settings.
Second-generation sequencing platforms such as Illumina have greatly expanded the …

Extended turnaround times and large economic costs hinder the usage of currently applied
screening methods for bacterial pathogen identification (ID) and antimicrobial susceptibility …

The prolonged incubation period of traditional culture methods leads to a delay in
diagnosing invasive infections. Nanopore 16S rRNA gene sequencing (Nanopore 16S) …

Metagenomic sequencing combined with Oxford Nanopore Technology has the potential to
become a point-of-care test for infectious disease in public health and clinical settings …