Objectives Lower respiratory tract infections (LRTIs) are one of the causes of mortality
among infectious diseases. Microbial cultures commonly used in clinical practice are time …

We report unbiased metagenomic detection of chikungunya virus (CHIKV), Ebola virus
(EBOV), and hepatitis C virus (HCV) from four human blood samples by MinION nanopore …

Background Nanopore metagenomics has been used for infectious disease diagnosis for
bacterial pathogens. However, this technology currently lacks comprehensive performance …

Recent advances in nanopore sequencing technology have led to a substantial increase in
throughput and sequence quality. Together, these improvements may permit real-time …

Objective To evaluate the value of nanopore targeted sequencing in diagnosing pneumonia
pathogens. Methods This large-scale multicentre prospective study performed in 8 hospitals …

US public health agencies have employed next-generation sequencing (NGS) as a tool to
quickly identify foodborne pathogens during outbreaks. Although established short-read …

While 16S rRNA PCR-Sanger sequencing has paved the way for the diagnosis of culture-
negative bacterial infections, it does not provide the composition of polymicrobial infections …

BACKGROUND As approximately 19% of global deaths are attributable to infectious
diseases, early diagnosis of infection is very important to reduce mortality. Traditional …

Rapid and accurate pathogen identification is crucial in effectively combating infectious
diseases. However, the current diagnostic tools for bacterial infections predominantly rely on …

Outbreak investigations are essential to control and prevent the dissemination of pathogens.
This study developed and validated a complete analysis protocol for faster and more …