Two‐hybrid analysis of domain interactions involving NtrB and NtrC two‐component regulators

I Martínez‐Argudo, J Martín‐Nieto… - Molecular …, 2001 - Wiley Online Library
Signal transduction by two‐component regulatory systems involves phosphorylation of the
receiver domain of a response regulator by the transmitter domain of the cognate histidine …

Domain Interactions on the ntr Signal Transduction Pathway: Two-Hybrid Analysis of Mutant and Truncated Derivatives of Histidine Kinase NtrB

I Martínez-Argudo, P Salinas, R Maldonado… - Journal of …, 2002 - Am Soc Microbiol
We have used the yeast two-hybrid system to analyze protein-protein interactions mediated
by domains of regulatory proteins of the ntr signal transduction system, including interactions …

Mechanism of activation of a response regulator: interaction of NtrC-P dimers induces ATPase activity

I Mettke, U Fiedler, V Weiss - Journal of bacteriology, 1995 - Am Soc Microbiol
NtrC is the transcriptional activator for nitrogen-regulated promoters and, as a response
regulator, belongs to the protein family of two-component systems. The activity of all …

Phosphorylation-independent dimer-dimer interactions by the enhancer-binding activator NtrC of Escherichia coli: a third function for the C-terminal domain

XF Yang, Y Ji, BL Schneider, L Reitzer - Journal of Biological Chemistry, 2004 - ASBMB
The response regulator NtrC transcriptionally activates genes of the nitrogen-regulated (Ntr)
response. Phosphorylation of its N-terminal receiver domain stimulates an essential …

Effector‐induced self‐association and conformational changes in the enhancer‐binding protein NTRC

ME Farez‐Vidal, TJ Wilson, BE Davidson… - Molecular …, 1996 - Wiley Online Library
The Klebsiella pneumoniae nitrogen regulatory protein NTRC is a response regulator which
activates transcription in response to nitrogen limitation, and is a member of the family of σN …

Substitutions at a single amino acid residue in the nitrogen‐regulated activator protein NTRC differentially influence its activity in response to phosphorylation

R Dixon, T Eydmann, N Henderson… - Molecular …, 1991 - Wiley Online Library
Four substitutions at serine residue 160 which increase the activity of the σ54‐dependent
activator protein NTRC in the absence of NTRB have been analysed in detail. Mutagenesis …

Analysis of the Klebsiella pneumoniae ntrB gene by site‐directed in vitro mutagenesis

SA MacFarlane, MJ Merrick - Molecular microbiology, 1987 - Wiley Online Library
A number of in‐frame insertion and deletion mutations have been constructed in vitro in the
Klebsiella pneumoniae ntrB gene and the effects of each mutant NtrB protein on NtrC activity …

Phosphorylation-induced signal propagation in the response regulator NtrC

J Lee, JT Owens, I Hwang, C Meares… - Journal of …, 2000 - Am Soc Microbiol
The bacterial enhancer-binding protein NtrC is a well-studied response regulator in a two-
component regulatory system. The amino (N)-terminal receiver domain of NtrC modulates …

Sequence of nifL from Klebsiella pneumoniae: mode of action and relationship to two families of regulatory proteins

MH Drummond1, JC Wootton - Molecular microbiology, 1987 - Wiley Online Library
We present the nucleotide sequence of K. pneumoniae nifL, which negatively regulates nif
transcription in response to oxygen and fixed nitrogen. It shows partial sequence homology …

The nucleotide sequence of the nitrogen regulation gene ntrB and the glnA-ntrBC intergenic region of Klebsiella pneumoniae

SA MacFarlane, M Merrick - Nucleic Acids Research, 1985 - academic.oup.com
The nucleotide sequence of the Klebsiella pneumoniae ntrB gene and the glnA-ntrBC
intergenic region has been determined. NtrB encodes a 38,409 Dalton polypeptide with a …