Transgenic animals are produced primarily by microinjecting exogenous DNA into the male
pronuclei of a zygote. Microinjection is successful in mice but not efficient in farm animals …

Since 1989, a new method for the production of transgenic animals has been available,
namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to …

“Sperm mediated gene transfer”(SMGT) is based on the ability of sperm cells to bind
exogenous DNA. The main objective of this study was to improve the production of …

Background Transgenic animals have become valuable tools for both research and applied
purposes. The current method of gene transfer, microinjection, which is widely used in …

Transgenic mammals have been produced using sperm as vectors for exogenous DNA
(sperm-mediated gene transfer (SMGT)) in combination with artificial insemination. Our …

Sperm-mediated gene transfer (SMGT) is a method for the production of transgenic animals
based on the intrinsic ability of sperm cells to bind and internalize exogenous DNA …

Ejaculated sperm cells from both bovine and swine animals depleted of the seminal fluid
were used as vectors for transferring different plasmid DNAs into eggs during the fertilization …

Current applications of gene transfer in farm animals include the improvement of product
quality and quantity, disease resistance, the production of valuable proteins in the mammary …

Transgenesis would be greatly streamlined if sperm cells could be used as transgene
vectors. Attempts to 'force'sperm cells to take up transgenes may be more worthy of further …

Sperm-mediated DNA transfer can be used to transfer exogenous DNA into the oocyte for
the production of transgenic animals. In spite of controversy in the literature, sperm-mediated …