Reduced lignin in transgenic plants containing a caffeic acidO-methyltransferase antisense gene

W Ni, NL Paiva, RA Dixon - Transgenic Research, 1994 - Springer
Lignin is a major structural polymer of secondarily thickended plant vascular tissue and
fibres, imparting mechanical strength to stems and trunks and hydrophobicity to conducting …

Modification of lignin biosynthesis in transgenic Nicotiana through expression of an antisense O-methyltransferase gene from Populus

UN Dwivedi, WH Campbell, J Yu, RSS Datla… - Plant Molecular …, 1994 - Springer
An aspen lignin-specific O-methyltransferase (bi-OMT; S-adenosyl-l-methionine: caffeic
acid/5-hydroxyferulic acid 3/5-O-methyltransferase, EC 2.1. 1.68) antisense sequence in the …

Simultaneous Down-Regulation of Caffeic/5-Hydroxy Ferulic Acid-O-Methyltransferase I and Cinnamoyl-Coenzyme A Reductase in the Progeny from a Cross …

G Pinçon, M Chabannes, C Lapierre, B Pollet… - Plant …, 2001 - academic.oup.com
Inhibition of specific lignin biosynthetic steps by antisense strategy has previously been
shown to alter lignin content and/or structure. In this work, homozygous tobacco (Nicotiana …

Altered lignin composition in transgenic tobacco expressing O‐methyltransferase sequences in sense and antisense orientation

R Atanassova, N Favet, F Martz, B Chabbert… - The Plant …, 1995 - Wiley Online Library
The monomeric composition of tobacco lignin has been modified by genetic engineering.
Sense or antisense expression of sequences encoding O‐methyltransferase (OMT), a lignin …

In situ analysis of lignins in transgenic tobacco reveals a differential impact of individual transformations on the spatial patterns of lignin deposition at the cellular and …

M Chabannes, K Ruel, A Yoshinaga… - The Plant …, 2001 - Wiley Online Library
Using tobacco transgenic lines altered in the monolignol biosynthetic pathway and which
differ in their lignin profiles we have evaluated lignin deposition at the cellular and …

Structural characterization of modified lignin in transgenic tobacco plants in which the activity of 4-coumarate: coenzyme A ligase is depressed

S Kajita, S Hishiyama, Y Tomimura… - Plant …, 1997 - academic.oup.com
Transgenic tobacco (Nicotiana tabacum L.) plants in which the activity of 4-coumarate:
coenzyme A ligase is very low contain a novel lignin in their xylem. Details of changes in …

Repression of O-methyltransferase genes in transgenic tobacco affects lignin synthesis and plant growth

G Pinçon, S Maury, L Hoffmann, P Geoffroy, C Lapierre… - Phytochemistry, 2001 - Elsevier
Among the different enzymatic steps leading to lignin biosynthesis, two methylation
reactions introduce the methyl groups borne by guaiacyl (G) and syringyl (S) units. Tobacco …

NMR characterization of altered lignins extracted from tobacco plants down-regulated for lignification enzymes cinnamylalcohol dehydrogenase and cinnamoyl-CoA …

J Ralph, RD Hatfield, J Piquemal… - Proceedings of the …, 1998 - National Acad Sciences
Homologous antisense constructs were used to down-regulate tobacco cinnamyl-alcohol
dehydrogenase (CAD; EC 1.1. 1.195) and cinnamoyl-CoA reductase (CCR; EC 1.2. 1.44) …

Fourier-transform infrared and Raman spectroscopic evidence for the incorporation of cinnamaldehydes into the lignin of transgenic tobacco (Nicotiana tabacum L.) …

D Stewart, N Yahiaoui, GJ McDougall, K Myton… - Planta, 1997 - Springer
Xylem from stems of genetically manipulated tobacco plants which had had cinnamyl
alcohol dehydrogenase (CAD; EC 1.1. 1.195) activity down-regulated to a greater or lesser …

Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa: Impacts on Lignin Structure and Implications for …

D Guo, F Chen, K Inoue, JW Blount, RA Dixon - The plant cell, 2001 - academic.oup.com
Transgenic alfalfa plants were generated harboring caffeic acid 3-O-methyltransferase
(COMT) and caffeoyl CoA 3-O-methyltransferase (CCOMT) cDNA sequences under control …