Design and construction of synthetic cellulosome with three adaptor scaffoldins for cellulosic ethanol production from steam-exploded corn stover

S Tian, JL Du, ZS Bai, J He, XS Yang - Cellulose, 2019 - Springer
In this study, a cellulosome-producing yeast reaction system with cellulase and
hemicellulase activities is developed for synergistic catalysis of steam-exploded corn stover …

In vitro assembly of minicellulosomes with two scaffoldins on the yeast cell surface for cellulose saccharification and bioethanol production

LH Fan, ZJ Zhang, XY Yu, YX Xue, MM Wang… - Process …, 2013 - Elsevier
The present paper reports in vitro strategies for assembly of minicellulosomes with two
miniscaffoldins on the Saccharomyces cerevisiae cell surface. It was carried out through …

[PDF][PDF] Direct ethanol production from steam-exploded corn stover using a synthetic diploid cellulase-displaying yeast consortium

C Mo, N Chen, T Lv, JL Du, S Tian - BioResources, 2015 - bioresources.cnr.ncsu.edu
The assembly of functional cellulolytic enzymes was displayed using a synthetic, cell-
surface engineered diploid yeast consortium. Trichoderma reesei endoglucanase II (EGII) …

Engineering yeast with bifunctional minicellulosome and cellodextrin pathway for co-utilization of cellulose-mixed sugars

LH Fan, ZJ Zhang, S Mei, YY Lu, M Li, ZY Wang… - Biotechnology for …, 2016 - Springer
Background Consolidated bioprocessing (CBP), integrating cellulase production, cellulose
saccharification, and fermentation into one step has been widely considered as the ultimate …

Consolidated bioprocessing for cellulosic ethanol conversion by cellulase–xylanase cell-surfaced yeast consortium

L Chen, JL Du, YJ Zhan, JA Li, RR Zuo… - Preparative …, 2018 - Taylor & Francis
Consolidated bioprocessing (CBP) strategy was developed to construct a cell-surface
displayed consortium for heterologously expressing functional lignocellulytic enzymes. The …

Cellulosic ethanol production using a yeast consortium displaying a minicellulosome and β-glucosidase

S Kim, SH Baek, K Lee, JS Hahn - Microbial Cell Factories, 2013 - Springer
Background Cellulosic biomass is considered as a promising alternative to fossil fuels, but
its recalcitrant nature and high cost of cellulase are the major obstacles to utilize this …

A novel integrated biological process for cellulosic ethanol production featuring high ethanol productivity, enzyme recycling and yeast cells reuse

M Jin, C Gunawan, N Uppugundla, V Balan… - Energy & …, 2012 - pubs.rsc.org
High enzyme loading requirements, slow xylose fermentation and low ethanol productivity
are three of the major issues impeding commercial biochemical production of cellulosic …

Engineered pentafunctional minicellulosome for simultaneous saccharification and ethanol fermentation in Saccharomyces cerevisiae

Y Liang, T Si, EL Ang, H Zhao - Applied and environmental …, 2014 - Am Soc Microbiol
Several yeast strains have been engineered to express different cellulases to achieve
simultaneous saccharification and fermentation of lignocellulosic materials. However …

Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production

Z Liu, SH Ho, K Sasaki, R Den Haan, K Inokuma… - Scientific reports, 2016 - nature.com
Cellulosic biofuel is the subject of increasing attention. The main obstacle toward its
economic feasibility is the recalcitrance of lignocellulose requiring large amount of enzyme …

A cellulosomal yeast reaction system of lignin-degrading enzymes for cellulosic ethanol fermentation

Y Ye, H Liu, Z Wang, Q Qi, J Du, S Tian - Biotechnology Letters, 2024 - Springer
Biofuel production from lignocellulose feedstocks is sustainable and environmentally
friendly. However, the lignocellulosic pretreatment could produce fermentation inhibitors …