DnaB helicases are bacterial, ATP‐driven enzymes that unwind double‐stranded DNA
during DNA replication. Herein, we study the sequential binding of the “non‐hydrolysable” …

Solid-state NMR spectroscopy has developed into a useful method for protein structure
determination. In contrast to diffraction methods, NMR spectroscopy does not rely on long …

DNP (dynamic nuclear polarization)-enhanced solid-state NMR is employed to directly
detect protein–DNA and protein–ATP interactions and identify the residue type establishing …

DnaB helicases are motor proteins that couple ATP-hydrolysis to the loading of the protein
onto DNA at the replication fork and to translocation along DNA to separate double-stranded …

Replicative helicases are major motor proteins essential for chromosomal DNA replication in
prokaryotes. Usually hexameric in solution, their DNA binding property must have different …

Paramagnetic metal ions can be inserted into ATP‐fueled motor proteins by exchanging the
diamagnetic Mg2+ cofactor with Mn2+ or Co2+. Then, paramagnetic relaxation …

The ATP hydrolysis transition state of motor proteins is a weakly populated protein state that
can be stabilized and investigated by replacing ATP with chemical mimics. We present …

Background: DnaB is the primary replicative helicase in Escherichia coli. Native DnaB is a
hexamer of identical subunits, each consisting of a larger C-terminal domain and a smaller …

Replication initiation is a crucial step in genome duplication and homohexameric DnaB
helicase plays a central role in the replication initiation process by unwinding the duplex …

Hexameric DnaB type replicative helicases are essential for DNA strand unwinding along
with the direction of replication fork movement. These helicases in general contain an amino …