INTRODUCTION A reference atlas of human cell types is a major goal for the field. Here, we
set out to generate single-cell atlases of both gene expression (this study) and chromatin …

The evolution of gene expression in mammalian organ development remains largely
uncharacterized. Here we report the transcriptomes of seven organs (cerebrum, cerebellum …

Animals display substantial inter-species variation in the rate of embryonic development
despite a broad conservation of the overall sequence of developmental events. Differences …

RNA abundance is a powerful indicator of the state of individual cells. Single-cell RNA
sequencing can reveal RNA abundance with high quantitative accuracy, sensitivity and …

During mammalian embryogenesis, differential gene expression gradually builds the identity
and complexity of each tissue and organ system. Here we systematically quantified mouse …

The formation of red blood cells begins with the differentiation of multipotent haematopoietic
progenitors. Reconstructing the steps of this differentiation represents a general challenge in …

Cell-fate transitions involve the integration of genomic information encoded by regulatory
elements, such as enhancers, with the cellular environment,. However, identification of …

Molecular profiling of single cells has advanced our knowledge of the molecular basis of
development. However, current approaches mostly rely on dissociating cells from tissues …

Pluripotent stem cells provide a powerful system to dissect the underlying molecular
dynamics that regulate cell fate changes during mammalian development. Here we report …

Embryonic stem (ES) cells can undergo many aspects of mammalian embryogenesis in
vitro,,,–, but their developmental potential is substantially extended by interactions with …