The prokaryote-derived CRISPR–Cas genome editing systems have transformed our ability
to manipulate, detect, image and annotate specific DNA and RNA sequences in living cells …

The discovery and subsequent repurposing of the CRISPR (clustered regularly interspaced
short palindromic repeats) and Cas proteins (CRISPR-associated proteins) has …

The CRISPR‐Cas technologies derived from bacterial and archaeal adaptive immune
systems have emerged as a series of groundbreaking nucleic acid‐guided gene editing …

CRISPR is becoming an indispensable tool in biological research. Once known as the
bacterial immune system against invading viruses, the programmable capacity of the Cas9 …

Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …

The CRISPR-Cas9 RNA-guided DNA endonuclease has contributed to an explosion of
advances in the life sciences that have grown from the ability to edit genomes within living …

Genome editing has enabled broad advances and novel approaches in studies of gene
function and structure; now, emerging methods aim to precisely engineer post …

The diversity, modularity, and efficacy of CRISPR-Cas systems are driving a
biotechnological revolution. RNA-guided Cas enzymes have been adopted as tools to …

The ability to add, remove, or change DNA sequences is essential to studies that investigate
the genetic underpinning of phenotypic traits. With its unprecedented efficiency and stunning …

A compact protein with a size of< 1,000 amino acids, the CRISPR-associated protein CasX
is a fundamentally distinct RNA-guided nuclease when compared to Cas9 and Cas12a …