ABSTRACT A sensitive multiplex PCR assay for single-tube amplification that detects
simultaneous herpes simplex virus type 1 (HSV-1), herpes simplex virus type 2 (HSV-2) …

We used a novel type of primer system, a system that uses stair primers, in which the primer
sequences are based on consensus sequences in the DNA polymerase gene of herpesvirus …

A total of 60 cerebrospinal fluid (CSF) specimens from patients manifesting symptoms
resembling viral central nervous system (CNS) disease were examined for the presence of …

Cerebrospinal fluid (CSF) specimens from 27 patients with encephalitis, meningitis, and
other neurological diseases were studied for the presence of herpes simplex virus types 1 …

Until recently, the laboratory diagnosis of central nervous system (CNS) infections with
herpes simplex virus (HSV) has been limited by poor sensitivity and/or specificity. We …

ABSTRACT A simultaneous detection system to quantify HSV, HHV‐6, and HHV‐7 DNA via
multiplex real‐time PCR using different fluorochromes was developed. The minimum …

We used a multiplex nested-PCR assay for the simultaneous detection in cerebrospinal fluid
(CSF) of five human herpesviruses (HVs)(cytomegalovirus [CMV], Epstein-Barr virus [EBV] …

Background: Infections of the central nervous system (CNS) are a difficult diagnostic
problem for both clinicians and microbiologists. Various clinical signs, such as encephalitis …

ABSTRACT A multiplex PCR assay that detects the four commonest causes of viral
meningitis and encephalitis in the United Kingdom (herpes simplex virus [HSV] type 1 [HSV …

Background: Herpesviruses may be associated with various types of central nervous system
(CNS) infections. Herpes simplex encephalitis (HSE) has to be considered one of the most …