Rapid progress in the development of next-generation sequencing (NGS) technologies in
recent years has provided many valuable insights into complex biological systems, ranging …

During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …

Single-cell transcriptomic technologies have emerged as powerful tools to explore cellular
heterogeneity at the resolution of individual cells. Previous scientific knowledge in cell …

Thanks to innovative sample-preparation and sequencing technologies, gene expression in
individual cells can now be measured for thousands of cells in a single experiment. Since its …

Heterogeneity in cell populations poses a significant challenge for understanding complex
cell biological processes. The analysis of cells at the single-cell level, especially single-cell …

Genome-wide single-cell analysis represents the ultimate frontier of genomics research. In
particular, single-cell RNA-sequencing (scRNA-seq) studies have been boosted in the last …

Cells are the basic building blocks of biological systems, with inherent unique molecular
features and development trajectories. The study of single cells facilitates in‐depth …

Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art
approach for unravelling the heterogeneity and complexity of RNA transcripts within …

Single-cell RNA sequencing (scRNA-seq) has provided a high-dimensional catalog of
millions of cells across species and diseases. These data have spurred the development of …

Highlights•Technologies such as single-cell RNA sequencing have rapidly progressed in
recent years.•Various methods exist for preparing libraries for sequencing, each with its own …