Instrumental and analysis improvements in multifrequency phase fluorometry

K Clays, J Jannes, Y Engelborghs… - Journal of Physics E …, 1989 - iopscience.iop.org
High-precision resolution for multi-exponential fluorescence decay is obtained by
completely automated multifrequency phase fluorometry, using the harmonic content of the …

What determines the uncertainty of phase and modulation measurements in frequency-domain fluorometry?

BB Barbieri, F De Piccoli… - Time-Resolved Laser …, 1990 - spiedigitallibrary.org
The basic theory of a frequency domain fluorometer has been known since the original work
of Gaviola (1)• With the introduction of multifrequency capabilities and the use of ultra high …

Recent developments in frequency-domain fluorometry

JR Lakowicz, BP Maliwal, E Gratton - 1985 - Taylor & Francis
I. SUMMARY Phase-modulation fluorometry is the frequency-domain analogue of time-
resolved fluorescence spectroscopy. During the past three years we witnessed the …

Construction and performance of a variable-frequency phase-modulation fluorometer

JR Lakowicz, BP Maliwal - Biophysical chemistry, 1985 - Elsevier
We describe the construction and performance of a variable-frequency phase-modulation
fluorometer. This instrument, which provides modulation frequencies from 1 to 200 MHz, was …

A continuously variable frequency cross-correlation phase fluorometer with picosecond resolution

E Gratton, M Limkeman - Biophysical journal, 1983 - cell.com
A detailed description of the construction and performance of a variable frequency cross-
correlation phase fluorometer is reported. The phase fluorometer operates over the …

Digital parallel acquisition in frequency domain fluorimetry

BA Feddersen, DW Piston, E Gratton - Review of scientific instruments, 1989 - pubs.aip.org
The analog electronics commonly found in frequency domain fluorometers are limited to
collecting only a single frequency at a time, and can be a source of systematic errors. We …

Resolution of mixtures of fluorophores using variable-frequency phase and modulation data

E Gratton, M Limkeman, JR Lakowicz, BP Maliwal… - Biophysical …, 1984 - cell.com
We measured fluorescence phase shift and modulation data for one-, two-and, three-
component mixtures of fluorophores at modulation frequencies ranging from 1 to 140 MHz …

A new fiber-optic-based multifrequency phase-modulation fluorometer

FV Bright - Applied spectroscopy, 1988 - opg.optica.org
A new fiber-optic-based fluorescence lifetime instrument is described which is useful for the
study of complex fluorescence decay kinetics. The new instrument consists of a …

[PDF][PDF] Comment on ''Digital parallel acquisition in frequency domain fluorometry''[Rev. Sci. Instrum. 6, 2929 (1989)]

JR Alcala - Review of Scientific Instruments, 1991 - pubs.aip.org
Feddersen et al. use pulsed cross-correlation techniques to obtain time resolved information
of fluorescence decays employing frequency domain techniques. While the implementation …

48000 MHF: a dual-domain Fourier transform fluorescence lifetime spectrofluorometer

GW Mitchell, KM Swift - Time-Resolved Laser Spectroscopy in …, 1990 - spiedigitallibrary.org
The technical simplicity and speed of cross-correlation frequency domain fluorometry has
been compromised recently by the need to measure and analyse the phase and modulation …