Interaction of purified NtrC protein with nitrogen regulated promoters from Klebsiella pneumoniae

T Hawkes, M Merrick, R Dixon - Molecular and General Genetics MGG, 1985 - Springer
The product of the Klebsiella pneumoniae nitrogen regulatory gene ntrC has been purified
and shown to be a dimeric protein of subunit molecular weight 54Kd, designated NtrC. In an …

Requirements for transcriptional activation in vitro of the nitrogen‐regulated gInA and nifLA promoters from Klebsiella pneumoniae: dependence on activator …

S Austin, N Henderson, R Dixon - Molecular microbiology, 1987 - Wiley Online Library
Three proteins involved in nitrogen regulation In Klebsiella pneumoniae, NTRA, NTRB and
NTRC, have been purified. In a defined in vitro system all three NTR proteins are required …

The nucleotide sequence of the nitrogen regulation gene ntrB and the glnA-ntrBC intergenic region of Klebsiella pneumoniae

SA MacFarlane, M Merrick - Nucleic Acids Research, 1985 - academic.oup.com
The nucleotide sequence of the Klebsiella pneumoniae ntrB gene and the glnA-ntrBC
intergenic region has been determined. NtrB encodes a 38,409 Dalton polypeptide with a …

Repressor properties of the nifL gene product in Klebsiella pneumoniae

M Merrick, S Hill, H Hennecke, M Hahn, R Dixon… - Molecular and General …, 1982 - Springer
Certain mutations in the nifL gene of the Klebsiella pneumoniae nitrogen fixation (nif) gene
cluster resulted in altered nif regulaiton such that nitrogenase synthesis was no longer …

Mutational analysis of the Klebsiella pneumoniae nitrogenase promoter: sequences essential for positive control by nifA and ntrC (glnG) products

DW Ow, Y Xiong, Q Gu, SC Shen - Journal of bacteriology, 1985 - Am Soc Microbiol
ntr (nitrogen regulated) and nif (nitrogen fixation) promoters are structurally similar to each
other but bear no resemblance to canonic Escherichia coli promoters. ntr promoters are …

Cloning of the glnA, ntrB and ntrC genes of Klebsiella pneumoniae and studies of their role in regulation of the nitrogen fixation (nif) gene cluster

G Espin, A Alvarez-Morales, F Cannon, R Dixon… - Molecular and General …, 1982 - Springer
The glanA, ntrB and ntrC genes of Klebsiella pneumoniae have been cloned, on a 12 kb
Hind III fragment, into the plasmid pACYC 184. In a coupled in vitro transcription/translation …

Sequence and domain relationships of ntrC and nifA from Klebsiella pneumoniae: homologies to other regulatory proteins.

M Drummond, P Whitty, J Wootton - The EMBO Journal, 1986 - embopress.org
We have determined the nucleotide sequences of two genes from Klebsiella pneumoniae,
nifA, the nif‐specific activator of transcription and ntrC, the bifunctional regulatory protein …

Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA.

MJ Merrick - The EMBO Journal, 1983 - embopress.org
The ntrC and nifA gene products of Klebsiella pneumoniae are transcriptional activators
involved in general nitrogen control and nif‐specific regulation, respectively. Multicopy …

In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae

PK Wong, D Popham, J Keener, S Kustu - Journal of bacteriology, 1987 - Am Soc Microbiol
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K.
pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an …

The effect on the function of the transcriptional activator NtrC from Klebsiella pneumoniae of mutations in the DNA-recognition helix

A Contreras, M Drummond - Nucleic acids research, 1988 - academic.oup.com
We have constructed mutations in what we predict to be the DNA-recognition helix of
Klebsiella pneumoniae NtrC, which regulates transcription from promoters under global …