A portable system for rapid bacterial composition analysis using a nanopore-based sequencer and laptop computer

S Mitsuhashi, K Kryukov, S Nakagawa, JS Takeuchi… - Scientific reports, 2017 - nature.com
We developed a portable system for 16S rDNA analyses consisting of a nanopore
technology-based sequencer, the MinION, and laptop computers, and assessed its potential …

Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer

S Kai, Y Matsuo, S Nakagawa, K Kryukov… - FEBS open …, 2019 - Wiley Online Library
Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic
treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene …

An identification protocol for ESBL-producing Gram-negative bacteria bloodstream infections using a MinION nanopore sequencer

J Sakai, N Tarumoto, M Kodana… - Journal of Medical …, 2019 - microbiologyresearch.org
Purpose. The new third-generation sequencing platform MinION is an attractive
maintenance-free and disposable portable tool that can perform long-read and real-time …

Evaluation of PCR conditions for characterizing bacterial communities with full-length 16S rRNA genes using a portable nanopore sequencer

S Fujiyoshi, A Muto-Fujita, F Maruyama - Scientific Reports, 2020 - nature.com
Abstract MinION (Oxford Nanopore Technologies), a portable nanopore sequencer, was
introduced in 2014 as a new DNA sequencing technology. MinION is now widely used …

DNA extraction of microbial DNA directly from infected tissue: an optimized protocol for use in nanopore sequencing

K Helmersen, HV Aamot - Scientific reports, 2020 - nature.com
Identification of bacteria causing tissue infections can be comprehensive and, in the cases of
non-or slow-growing bacteria, near impossible with conventional methods. Performing …

Rapid identification of pathogens from positive blood culture bottles with the MinION nanopore sequencer

S Ashikawa, N Tarumoto, K Imai… - Journal of Medical …, 2018 - microbiologyresearch.org
Purpose. Bloodstream infections are major causes of morbidity and mortality that lead to
prolonged hospital stays and higher medical costs. In this study, we aimed to evaluate the …

Third-generation sequencing in the clinical laboratory: exploring the advantages and challenges of nanopore sequencing

LM Petersen, IW Martin, WE Moschetti… - Journal of clinical …, 2019 - Am Soc Microbiol
Metagenomic sequencing for infectious disease diagnostics is an important tool that holds
promise for use in the clinical laboratory. Challenges for implementation so far include high …

Off earth identification of bacterial populations using 16S rDNA nanopore sequencing

AS Burton, SE Stahl, KK John, M Jain, S Juul… - Genes, 2020 - mdpi.com
The MinION sequencer has made in situ sequencing feasible in remote locations. Following
our initial demonstration of its high performance off planet with Earth-prepared samples, we …

Evaluation of Oxford Nanopore's MinION sequencing device for microbial whole genome sequencing applications

AD Tyler, L Mataseje, CJ Urfano, L Schmidt… - Scientific reports, 2018 - nature.com
Abstract The MinION sequencer (Oxford Nanopore Technologies) is a paradigm shifting
device allowing rapid, real time long read sequencing of nucleic acids. Yet external …

Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution

Y Matsuo, S Komiya, Y Yasumizu, Y Yasuoka… - BMC microbiology, 2021 - Springer
Background Species-level genetic characterization of complex bacterial communities has
important clinical applications in both diagnosis and treatment. Amplicon sequencing of the …