Fluorescence bioimaging has recently jumped into a new area of spatiotemporal resolution
and sensitivity thanks to synergistic advances in both optical physics and probe/biosensor …

Our ability to observe biochemical events with high spatial and temporal resolution is
essential for understanding the functioning of living systems. Intrinsically fluorescent proteins …

Cellular activity is defined by the precise spatiotemporal regulation of various components,
such as ions, small molecules, or proteins. Studying cell physiology consequently requires …

Optical fluorescence microscopy plays a pivotal role in the exploration of biological structure
and dynamics, especially on live specimens. Progress in the field relies, on the one hand, on …

Inducible chemical-genetic fluorescent markers are promising tools for live cell imaging
requiring high spatiotemporal resolution and low background fluorescence. The …

Highlights•New fluorophores with optimised photophysical properties for in vivo
imaging.•Near-infrared-emitting fluorophore scaffolds that can be modularly functionalised.• …

The spatiotemporal fluorescence imaging of biological processes requires effective tools to
label intracellular biomolecules in living systems. This review presents a brief overview of …

One of the major challenges of modern biology and medicine consists in finding means to
visualize biomolecules in their natural environment with the greatest level of accuracy, so as …

Fluorogenic molecules are important tools for biological and biochemical research. The
majority of fluorogenic compounds have a simple input–output relationship, where a single …

With the escalation of drug discovery programmes, it has become essential to visualize and
monitor biological activities in healthy and pathological cells, with high spatial and temporal …