Dynamic control of enzyme expression can be an effective strategy to engineer robust
metabolic pathways. It allows a synthetic pathway to self-regulate in response to changes in …

Synthetic biology combines different branches of biology and engineering aimed at
designing synthetic biological circuits able to replicate emergent properties useful for the …

A grand challenge in synthetic biology is to push the design of biomolecular circuits from
purely genetic constructs towards systems that interface different levels of the cellular …

Inside individual cells, protein population counts are subject to molecular noise due to low
copy numbers and the inherent probabilistic nature of biochemical processes. We …

Background Noise has many important roles in cellular genetic regulatory functions at the
nanomolar scale. At present, no good theory exists for identifying all possible mechanisms of …

We describe a frequency domain technique for the analysis of intrinsic noise within
negatively autoregulated gene circuits. This approach is based on the transfer function …

Cellular metabolism contains intricate arrays of feedback control loops. These are a key
mechanism by which cells adapt and survive environmental disturbances. Gene regulation …

Microorganisms (mainly bacteria and yeast) are frequently used as hosts for genetic
constructs in synthetic biology applications. Molecular noise might have a significant effect …

Synthetic gene networks can be used to control gene expression and cellular phenotypes in
a variety of applications. In many instances, however, such networks can behave unreliably …

We propose a negative feedback architecture that regulates activity of artificial genes, or
“genelets”, to meet their output downstream demand, achieving robustness with respect to …