The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S
rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA …

Background DNA extraction is an important factor influencing the microbiome profile in fecal
samples. Considering that the QIAamp DNA Stool Mini Kit, one of the most commonly used …

The analysis of microbiota composition in humans, animals, and built environments is
important because of emerging roles and applications in a broad range of disease and …

The human gut harbors a vast range of microbes that have significant impact on health and
disease. Therefore, gut microbiome profiling holds promise for use in early diagnosis and …

Many studies correlate changes in human gut microbiome with the onset of various
diseases, mostly by 16S rRNA gene sequencing. Setting up the optimal sampling and DNA …

Background Metagenomics seeks to understand microbial communities and assemblages
by DNA sequencing. Technological advances in next generation sequencing technologies …

The human microbiome has emerged as a major player in regulating human health and
disease. Translational studies of the microbiome have the potential to indicate clinical …

Culture-independent methods have granted the possibility to study microbial diversity in
great detail, but technical issues pose a threat to the accuracy of new findings. Biases …

The advent of affordable high-throughput DNA sequencing has opened up a golden age of
studies in the human microbiome. In order to understand the role of the human microbiota …

Several different protocols are used for fecal DNA extraction, which is an integral step in all
phylogenetic and metagenomic approaches to characterize the highly diverse intestinal …