Validation of a Fluorescence In Situ Hybridization Method Using Peptide Nucleic Acid Probes for Detection of Helicobacter pylori Clarithromycin Resistance in Gastric …

L Cerqueira, RM Fernandes, RM Ferreira… - Journal of Clinical …, 2013 - Am Soc Microbiol
Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ
hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori …

[HTML][HTML] PCR detection of clarithromycin-susceptible and-resistant Helicobacter pylori from formalin-fixed, paraffin-embedded gastric biopsies

BH Schmitt, MA Regner, KA Mangold, RB Thomson Jr… - Modern pathology, 2013 - Elsevier
Antimicrobial resistance to clarithromycin is a growing concern in the treatment of
Helicobacter pylori and is associated with three major point mutations of the 23S rRNA …

PCR-Based Diagnosis of Helicobacter pylori Infection and Real-Time Determination of Clarithromycin Resistance Directly from Human Gastric Biopsy Samples

SA Chisholm, RJ Owen, EL Teare… - Journal of Clinical …, 2001 - Am Soc Microbiol
ABSTRACT A novel PCR detection assay that amplifies the Helicobacter pylori-specific
vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is …

Fast and Accurate Quantitative Detection of Helicobacter pylori and Identification of Clarithromycin Resistance Mutations in H. pylori Isolates from Gastric Biopsy …

C Lascols, D Lamarque, JM Costa… - Journal of clinical …, 2003 - Am Soc Microbiol
Rapid identification of patients infected with clarithromycin-resistant Helicobacter pylori
without the need for culture can help to avoid useless prescriptions of clarithromycin. We …

Rapid and specific detection of Helicobacter pylori macrolide resistance in gastric tissue by fluorescent in situ hybridisation

K Trebesius, K Panthel, S Strobel, K Vogt, G Faller… - Gut, 2000 - gut.bmj.com
BACKGROUND The development of macrolide resistance in Helicobacter pylori is
considered an essential reason for failure of antibiotic eradication therapies. The …

PNA-FISH as a new diagnostic method for the determination of clarithromycin resistance of Helicobacter pylori

L Cerqueira, RM Fernandes, RM Ferreira, F Carneiro… - BMC microbiology, 2011 - Springer
Background Triple therapy is the gold standard treatment for Helicobacter pylori eradication
from the human stomach, but increased resistance to clarithromycin became the main factor …

Comparison of Fluorescent In Situ Hybridization and Conventional Culturing for Detection of Helicobacter pylori in Gastric Biopsy Specimens

H Rüssmann, VAJ Kempf, S Koletzko… - Journal of Clinical …, 2001 - Am Soc Microbiol
In this study, we have investigated 201 gastric biopsy specimens obtained from dyspeptic
patients for the presence of Helicobacter pylori. By means of fluorescent in situ hybridization …

Detection of Helicobacter pylori and determination of clarithromycin susceptibility using formalin‐fixed, paraffin‐embedded gastric biopsy specimens by fluorescence …

Ö Yilmaz, E Demiray, S Tümer, O Altungöz… - …, 2007 - Wiley Online Library
Background: Clarithromycin resistance and poor compliance to therapy are often
responsible for Helicobacter pylori eradication therapy failure. Aim: To evaluate …

[HTML][HTML] Real-time PCR for Helicobacter pylori quantification and detection of clarithromycin resistance in gastric tissue from patients with gastrointestinal disorders

M Kargar, S Ghorbani-Dalini, A Doosti… - Research in microbiology, 2012 - Elsevier
Helicobacter pylori gram-negative bacteria commonly infect the human gastrointestinal (GI)
tract and are readily diagnosed by endoscopy. H. pylori infection causes a broad range of …

Molecular detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies: a prospective evaluation of RIDA®GENE Helicobacter pylori assay

B Van den Poel, S Gils, I Micalessi, S Carton… - Acta Clinica …, 2021 - Taylor & Francis
ABSTRACT Background: Empirical treatment of Helicobacter pylo ri (HP) depends on the
local prevalence of clarithromycin resistance but data are lacking and culturing of HP is time …