Many studies correlate changes in human gut microbiome with the onset of various
diseases, mostly by 16S rRNA gene sequencing. Setting up the optimal sampling and DNA …

The gut microbiome is widely analyzed using high-throughput sequencing, such as 16S
rRNA gene amplicon sequencing and shotgun metagenomic sequencing (SMS). DNA …

The composition of human as well as animal microbiota has increasingly gained in interest
since metabolites and structural components of endogenous microorganisms fundamentally …

Microbial metagenomics are hindered in clinical tissue samples as a result of the large
relative amount of human DNA in relation to microbial DNA acting as competitive inhibitors …

Introduction Determining bacterial community structure in fecal samples through DNA
sequencing is an important facet of intestinal health research. The impact of different …

Culture-independent methods have granted the possibility to study microbial diversity in
great detail, but technical issues pose a threat to the accuracy of new findings. Biases …

The human gut contains dense and diverse microbial communities which have profound
influences on human health. Gaining meaningful insights into these communities requires …

Next-generation sequencing (NGS) is currently the method of choice for analyzing gut
microbiota composition. As gut microbiota composition is a potential future target for clinical …

Our understanding of human gut microbiota in health and disease depends on accurate and
reproducible microbial data acquisition. The critical step in this process is to apply an …

The human gut harbors a vast range of microbes that have significant impact on health and
disease. Therefore, gut microbiome profiling holds promise for use in early diagnosis and …