Electron tomography simulator with realistic 3D phantom for evaluation of acquisition, alignment and reconstruction methods

X Wan, T Katchalski, C Churas, S Ghosh… - Journal of structural …, 2017 - Elsevier
Because of the significance of electron microscope tomography in the investigation of
biological structure at nanometer scales, ongoing improvement efforts have been …

Automated procedures for the alignment and reconstruction of multiple tilt electron microscopic tomography data

MH Ellisman, D Boassa, P Nguyen, X Wan… - Microscopy and …, 2014 - cambridge.org
Transmission electron microscopy, combined with tomographic techniques, has been used
for decades to generate 3D representations of biological structures, with resolutions down to …

3D reconstruction of biological structures: automated procedures for alignment and reconstruction of multiple tilt series in electron tomography

S Phan, D Boassa, P Nguyen, X Wan, J Lanman… - Advanced structural and …, 2016 - Springer
Transmission electron microscopy allows the collection of multiple views of specimens and
their computerized three-dimensional reconstruction and analysis with electron tomography …

Segmentation of features in electron tomographic reconstructions

N Volkmann - Cellular Imaging: Electron Tomography and Related …, 2018 - Springer
Electron tomography is the most widely applicable method for obtaining three-dimensional
information from biological samples by electron microscopy. However, owing to the …

The nuts and bolts of electron tomography: Imaging of big and messy biological structures

GE Sosinsky - Microscopy and Microanalysis, 2002 - cambridge.org
Proteomics has been described as the attempt to capture the dynamics of a living system
from the collection of proteins that are up and down regulated during a cell's lifetime [1] …

[PDF][PDF] An introduction to low dose electron tomography-from specimen preparation to data collection

G Owen, DL Stokes - Modern Research and Educational Topics in …, 2007 - researchgate.net
Electron tomography is a technique that uses a Transmission Electron Microscope (TEM) to
determine a three-dimensional (3D) structure from any given asymmetric object [1]. This …

A Novel Method for Automated Acquisition of Tilt Series for Electron Tomography Based on Pre-Calibration of the Specimen Stage

U Ziese, AH Janssen, TP van der Krift… - Microscopy and …, 2000 - cambridge.org
Electron tomography is a three-dimensional (3D) imaging method with transmission electron
microscopy (TEM) that provides high-resolution 3D images of structural arrangements …

Electron tomography—a tool for ultrastructural 3D visualization in cell biology and histology

J Neumüller - Wiener Medizinische Wochenschrift, 2018 - Springer
Electron tomography (ET) was developed to overcome some of the problems associated
reconstructing three-dimensional (3D) images from 2D election microscopy data from …

A joint alignment and reconstruction algorithm for electron tomography to visualize in-depth cell-to-cell interactions

L Bogensperger, E Kobler, D Pernitsch… - Histochemistry and Cell …, 2022 - Springer
Electron tomography allows one to obtain 3D reconstructions visualizing a tissue's
ultrastructure from a series of 2D projection images. An inherent problem with this imaging …

Principles and practice in electron tomography

BF McEwen, C Renken, M Marko, C Mannella - Methods in cell biology, 2008 - Elsevier
Publisher Summary In electron tomography, a three-dimensional (3D) image is
reconstructed from 2D projection images, usually by back-projection methods. The same …