G proteins play a vital role in fundamental signal transduction events, from vision to smell, to
neurotransmitter and hormonal activities. 1 In their inactive state (State 1 in Fig. 1), they exist …

We study wild type and mutants of the A and B domain of protein G using all-atom Go-
models. Our data substantiate the usefulness of such simulation for probing the folding …

The solution structure of the human protein GAIP (Gα interacting protein), a regulator of G
protein signaling, has been determined by NMR techniques. Dipolar couplings of the …

1. Introduction 22. Heterotrimeric G-protein structure 32.1. G-protein α subunit 32.2. G-
protein βγ dimer 82.3. Unique role of Gβ5 in complexes with RGS proteins 92.4 …

Heterotrimeric guanine-nucleotide-binding regulatory proteins (G-proteins) mainly relay the
information from G-protein-coupled receptors (GPCRs) on the plasma membrane to the …

Heterotrimeric G proteins are conformational switches that turn on intracellular signaling
cascades in response to the activation of G-protein-coupled receptors. Receptor activation …

To understand the dynamics of conformational changes during G protein activation, surface
exposed cysteine residues on Gα were fluorescently labeled. Limited trypsinolysis and …

Heterotrimeric G protein α (Gα) subunits possess intrinsic GTPase activity that leads to
functional deactivation with a rate constant of≈ 2 min-1 at 30° C. GTP hydrolysis causes …

The heterotrimeric G proteins (Gαβγ) act as molecular switches to mediate signal
transduction from G protein-coupled receptors to downstream effectors. Upon interaction …

GAIP is a regulator of G protein signaling (RGS) that accelerates the rate of GTP hydrolysis
by some G protein α subunits. In the present studies, we have examined the structural basis …