In vivo microscopy is a powerful method for studying fundamental issues of physiology and
pathophysiology. The recent development of multiphoton fluorescence microscopy has …

The use of multiphoton microscopy for imaging mouse brain in vivo offers several
advantages and poses several challenges. This tutorial begins by briefly comparing …

Multiphoton microscopy is based on the simultaneous absorption of two photons emitted by
a pulsed infrared laser source. In this technique, the excitation is restricted to a very small …

Since its invention 29 years ago, two‐photon laser‐scanning microscopy has evolved from a
promising imaging technique, to an established widely available imaging modality used …

This chapter provides an overview of basic principles of multiphoton microscopy, including a
brief summary of how multiphoton microscopy fits within the arena of fluorescent microscopy …

Two‐photon excitation microscopy is an alternative to confocal microscopy that provides
advantages for three‐dimensional and deep tissue imaging. This unit will describe the basic …

Since the first use of multiphoton excitation for fluorescence microscopy (Denk et al., 1990)
the technique has gained acceptance as the preferred method of imaging biological …

Leaps in scientific technology often occur at the interface of seemingly disparate disciplines.
This holds true with the recent application of multiphoton microscopy to the biological …

We present a review of imaging deep-tissue structures with multiphoton microscopy. We
examine the effects of light scattering and absorption due to the optical properties of …

Publisher Summary This chapter describes in vivo and ex vivo tissue applications of two-
photon microscopy (TPM) and reviews key areas—such as neurobiology—in which TPM …