Simultaneous suppression of multiple genes by single transgenes. Down-regulation of three unrelated lignin biosynthetic genes in tobacco
JC Abbott, A Barakate, G Pinçon, M Legrand… - Plant …, 2002 - academic.oup.com
Many reports now describe the manipulation of plant metabolism by suppressing the
expression of single genes. The potential of such work could be greatly expanded if multiple …
expression of single genes. The potential of such work could be greatly expanded if multiple …
Enabling technologies for manipulating multiple genes on complex pathways
Many complex biochemical pathways in plants have now been manipulated genetically,
usually by suppression or over-expression of single genes. Further exploitation of the …
usually by suppression or over-expression of single genes. Further exploitation of the …
High-throughput vectors for efficient gene silencing in plants
CA Helliwell, SV Wesley, AJ Wielopolska… - Functional plant …, 2002 - CSIRO Publishing
A major challenge in the post-genome era of plant biology is to determine the functions of all
genes in the plant genome. A straightforward approach to this problem is to reduce or …
genes in the plant genome. A straightforward approach to this problem is to reduce or …
Stacking transgenes in forest trees
C Halpin, W Boerjan - Trends in Plant Science, 2003 - cell.com
Huge potential exists for improving plant raw materials and foodstuffs via metabolic
engineering. To date, progress has mostly been limited to modulating the expression of …
engineering. To date, progress has mostly been limited to modulating the expression of …
Constructs and methods for high-throughput gene silencing in plants
C Helliwell, P Waterhouse - Methods, 2003 - Elsevier
Gene silencing can be achieved by transformation of plants with constructs that express self-
complementary (termed hairpin) RNA containing sequences homologous to the target …
complementary (termed hairpin) RNA containing sequences homologous to the target …
Plant gene silencing regularized
G Bruening - Proceedings of the National Academy of …, 1998 - National Acad Sciences
Two surprising and potentially useful phenomena of transgenic plants originated from two
distinct lines of research but may converge in underlying mechanism or mechanisms (1–3) …
distinct lines of research but may converge in underlying mechanism or mechanisms (1–3) …
Transgene constructs lacking transcription termination signal induce efficient silencing of endogenous targets in Arabidopsis
SJ Nicholson, V Srivastava - Molecular Genetics and Genomics, 2009 - Springer
Efficient gene silencing tools are extremely important for genomics and biotechnology
applications. A number of aberrant RNA species induce silencing of homologous genes; …
applications. A number of aberrant RNA species induce silencing of homologous genes; …
Modification of lignin biosynthesis in transgenic Nicotiana through expression of an antisense O-methyltransferase gene from Populus
UN Dwivedi, WH Campbell, J Yu, RSS Datla… - Plant Molecular …, 1994 - Springer
An aspen lignin-specific O-methyltransferase (bi-OMT; S-adenosyl-l-methionine: caffeic
acid/5-hydroxyferulic acid 3/5-O-methyltransferase, EC 2.1. 1.68) antisense sequence in the …
acid/5-hydroxyferulic acid 3/5-O-methyltransferase, EC 2.1. 1.68) antisense sequence in the …
Temporal and spatial control of gene silencing in transgenic plants by inducible expression of double‐stranded RNA
Downregulation of endogenous genes via post‐transcriptional gene silencing (PTGS) is a
key to the characterization of gene function in plants. The recent discovery that double …
key to the characterization of gene function in plants. The recent discovery that double …
Reduced lignin in transgenic plants containing a caffeic acidO-methyltransferase antisense gene
W Ni, NL Paiva, RA Dixon - Transgenic Research, 1994 - Springer
Lignin is a major structural polymer of secondarily thickended plant vascular tissue and
fibres, imparting mechanical strength to stems and trunks and hydrophobicity to conducting …
fibres, imparting mechanical strength to stems and trunks and hydrophobicity to conducting …