The complexity of gene regulatory networks that lead multipotent cells to acquire different
cell fates makes a quantitative understanding of differentiation challenging. Using a …

Gene expression heterogeneity in the pluripotent state of mouse embryonic stem cells
(mESCs) has been increasingly well-characterized. In contrast, exit from pluripotency and …

We demonstrate the qualitative and quantitative power of single-cell transcript analysis to
characterize transcriptome dynamics in human embryonic stem cells (hESC's). Single-cell …

Investigating the molecular mechanisms controlling the in vivo developmental program
postembryogenesis is challenging and time consuming. However, the developmental …

Background Human pluripotent stem cells offer the best available model to study the
underlying cellular and molecular mechanisms of human embryonic lineage specification …

During in vitro differentiation, pluripotent stem cells undergo extensive remodeling of their
gene expression profile. While studied extensively at the transcriptome level, much less is …

Single-cell profiling techniques create opportunities to delineate cell fate progression in
mammalian development. Recent studies have provided transcriptome data from human pre …

Stem cell differentiation is a highly dynamic process involving pervasive changes in gene
expression. The large majority of existing studies has characterized differentiation at the …

During the transition from the inner cell mass (ICM) cells of blastocysts to pluripotent
embryonic stem cells (ESCs) in vitro, a normal developmental program is replaced in cells …

Cell fate decisions play a pivotal role in development, but technologies for dissecting them
are limited. We developed a multifunction new method, Topographer, to construct a …