Mutational analysis of upstream sequences required for transcriptional activation of the Klebsiella pneumoniae nifH promoter

M Buck, W Cannon, J Woodcock - Nucleic acids research, 1987 - academic.oup.com
Upstream sequences of the Klebsiella pneumoniae nifH promoter were mutagenised and
activation of the mutated promoters by the nif-specific transcriptional activator protein NifA …

Positional requirements for the function of nif-specific upstream activator sequences

M Buck, J Woodcock, W Cannon, L Mitchenall… - Molecular and General …, 1987 - Springer
The upstream activator sequence (UAS) found in Klebsiella pneumoniae nif promoters and
required for the activation of transcription by nifA, is absent from the nifF-nifL intergenic …

Mutations in the RNA polymerase recognition sequence of the Klebsiella pneumoniae nifH promoter permitting transcriptional activation in the absence …

M Buck, W Cannon - Nucleic acids research, 1989 - academic.oup.com
Positive control of the wild-type Klebsiella pneumoniae nifH promoter by the NifA protein
requires that NifA is bound at the upstream activator sequence (UAS). By introducing base …

The role of activator binding sites in transcriptional control of the divergently transcribed nifF and nif LA promoters from Klebsiella pneumoniae

SD Minchin, S Austin, RA Dixon - Molecular microbiology, 1988 - Wiley Online Library
The regulatory region spanning the divergently transcribed nif F and nifLA promoters
contains a NIFA‐specific upstream activator sequence (UAS) located around+ 59, and two …

The influence of the Klebsiella pneumoniae regulatory gene nifL upon the transcriptional activator protein NifA

E Morett, R Kreutzer, W Cannon… - Molecular …, 1990 - Wiley Online Library
The influence of the Klebsiella pneumoniae nifL gene product upon the interaction of the
transcriptional activator protein NifA with the nifH promoter has been examined using in vivo …

Site-directed mutagenesis of the Klebsiella pneumoniae nifL and nifH promoters and in vivo analysis of promoter activity

M Buck, H Khan, R Dixon - Nucleic Acids Research, 1985 - academic.oup.com
The role of conserved nucleotides in nitrogen-fixation promoter function has been examined
using both oligonucleotide and chemical mutagenesis to introduce base changes in the …

Deletion loop mutagenesis of the nifL promoter from Klebsiella pneumoniae: role of the-26 to-12 region in promoter function

H Khan, M Buck, R Dixon - Gene, 1986 - Elsevier
Nine single C-to-T transitions were introduced into the-26 to-12 region of the Klebsiella
pneumoniae nifL promoter by bisulphite mutagenesis of M 13 heteroduplexes containing a …

Organization and function of binding sites for the transcriptional activator NifA in the Klebsiella pneumoniae nifE and nifU promoters

W Cannon, W Charlton, M Buck - Journal of molecular biology, 1991 - Elsevier
The interaction of the Klebsiella pneumoniae NifA protein, a σ 54-dependent activator, with
the nifE and nifU promoters was analysed. At these promoters NifA established contacts in …

Activation of the Klebsiella pneumoniae nifU promoter: identification of multiple and overlapping upstream NifA binding sites

WV Cannon, R Kreutzer, HM Kent… - Nucleic acids …, 1990 - academic.oup.com
The Klebsiella pneumoniae nifU promoter is positively controlled by the NifA protein and
requires a form of RNA polymerase holoenzyme containing the rpoN encoded sigma factor …

The Klebsiella pneumoniae nifJ promoter: analysis of promoter elements regulating activation by the Nif A promoter

W Charlton, W Cannon, M Buck - Molecular microbiology, 1993 - Wiley Online Library
The nifJ and nifH promoters of Klebsiella pneumoniae are divergently transcribed σ; 54‐
dependent promoters that are positively activated by the NifA protein. NifA binds to upstream …