Cre and FLP recombinases are the most widely used site-specific recombinases in genome
engineering. Both are members of the tyrosine class of recombinases and catalyze the …

Plasmids were constructed carrying the loxP and FRT recognition sites for the Cre and Flp
site-specific recombinases, respectively, within multiple cloning sites. Vectors carrying single …

We developed two new site-specific recombination systems named VCre/VloxP and
SCre/SloxP for genome engineering. Their recognition sites are different from Cre …

DNA site-specific recombinases (SSRs) such as Cre, FLPe, and φC31, are powerful tools for
analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs …

We report the construction of two Escherichia coli strains (294-Cre and 294-FLP) which
express either Cre-or FLP-recombinase. Plasmids containing authentic recognition targets …

The site-specific recombinases FLP and Cre are useful for genomic engineering in many
living systems. Manipulation of their enzymatic properties offers a means to improve their …

Site-specific recombinase systems (Cre-loxP, Flp-FRT, and φC31-att) are transforming both
forward and reverse genetics in mice. By enabling high-fidelity DNA modifications to be …

Recombinases derived from microorganisms mediate efficient site-specific recombination.
For example, the Cre recombinase from bacteriophage P1 efficiently carries out …

Manipulation of gene expression patterns through gene targeting in mouse embryonic stem
(ES) cells (Thomas and Capecchi, 1987) and the more recent advent of conditional …

Genome engineering relies on DNA‐modifying enzymes that are able to locate a DNA
sequence of interest and initiate a desired genome rearrangement. Currently, the field …