Mechanism of transcriptional activation by NtrC

SC Porter, AK North, S Kustu - Two‐Component Signal …, 1995 - Wiley Online Library
In the case of nitrogen regulatory protein C (NtrC), both nucleotide hydrolysis and
transcriptional activation depend on phosphorylation of an aspartate residue in the N …

The major dimerization determinants of the nitrogen regulatory protein NTRC from enteric bacteria lie in its carboxy-terminal domain

KE Klose, AK North, KM Stedman, S Kustu - Journal of molecular biology, 1994 - Elsevier
The NTRC protein (nitrogen regulatory protein C) of enteric bacteria is an enhancer-binding
protein that activates transcription by the σ 54-holoenzyme form of RNA polymerase. NTRC …

Constitutive forms of the enhancer-binding protein NtrC: evidence that essential oligomerization determinants lie in the central activation domain

Y Flashner, DS Weiss, J Keener, S Kustu - Journal of molecular biology, 1995 - Elsevier
Nitrogen regulatory protein C (NtrC) is a bacterial enhancer-binding protein that activates
transcription by the σ54-holoenzyme. To activate transcription, NtrC must hydrolyze ATP, a …

Oligomerization of NTRC at the glnA enhancer is required for transcriptional activation.

SC Porter, AK North, AB Wedel… - Genes & …, 1993 - genesdev.cshlp.org
To activate transcription of the glnA gene, the dimeric NTRC protein (nitrogen regulatory
protein C) of enteric bacteria binds to an enhancer located approximately 100 bp upstream …

Repressor forms of the enhancer-binding protein NtrC: some fail in coupling ATP hydrolysis to open complex formation by σ54-holoenzyme

AK North, DS Weiss, H Suzuki, Y Flashner… - Journal of molecular …, 1996 - Elsevier
NtrC (nitrogen regulatory protein C) is a bacterial enhancer-binding protein that activates
transcription by catalyzing isomerization of closed complexes between σ54-holoenzyme and …

The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription

DS Weiss, J Batut, KE Klose, J Keener, S Kustu - Cell, 1991 - cell.com
The NTRC protein of enteric bacteria is an enhancerbinding protein that activates
transcription in response to limitation of combined nitrogen. NTRC activates transcription by …

Physical evidence for a phosphorylation-dependent conformational change in the enhancer-binding protein NtrC

I Hwang, T Thorgeirsson, J Lee… - Proceedings of the …, 1999 - National Acad Sciences
The bacterial enhancer-binding protein nitrogen regulatory protein C (NtrC) activates
transcription by σ54-containing RNA polymerase in a reaction that depends on ATP …

Unusual oligomerization required for activity of NtrC, a bacterial enhancer-binding protein

C Wyman, I Rombel, AK North, C Bustamante, S Kustu - Science, 1997 - science.org
Nitrogen regulatory protein C (NtrC) contacts a bacterial RNA polymerase from distant
enhancers by means of DNA loops and activates transcription by allowing polymerase to …

“Switch I” mutant forms of the bacterial enhancer-binding protein NtrC that perturb the response to DNA

D Yan, S Kustu - Proceedings of the National Academy of …, 1999 - National Acad Sciences
NtrC (nitrogen regulatory protein C) is a bacterial enhancer-binding protein of 469 residues
that activates transcription by σ54-holoenzyme. A region of its transcriptional activation …

Mechanism of activation of a response regulator: interaction of NtrC-P dimers induces ATPase activity

I Mettke, U Fiedler, V Weiss - Journal of bacteriology, 1995 - Am Soc Microbiol
NtrC is the transcriptional activator for nitrogen-regulated promoters and, as a response
regulator, belongs to the protein family of two-component systems. The activity of all …