SPICE: exploration and analysis of post‐cytometric complex multivariate datasets

M Roederer, JL Nozzi, MC Nason - Cytometry Part A, 2011 - Wiley Online Library
Polychromatic flow cytometry results in complex, multivariate datasets. To date, tools for the
aggregate analysis of these datasets across multiple specimens grouped by different …

Automated high-dimensional flow cytometric data analysis

S Pyne, X Hu, K Wang, E Rossin… - Proceedings of the …, 2009 - National Acad Sciences
Flow cytometric analysis allows rapid single cell interrogation of surface and intracellular
determinants by measuring fluorescence intensity of fluorophore-conjugated reagents. The …

Nine‐color flow cytometry for accurate measurement of T cell subsets and cytokine responses. Part I: Panel design by an empiric approach

BE McLaughlin, N Baumgarth, M Bigos… - Cytometry Part A: the …, 2008 - Wiley Online Library
Polychromatic flow cytometry offers the unprecedented ability to investigate multiple
antigens per cell. Unfortunately, unwanted spectral overlaps and compensation problems …

The end of gating? An introduction to automated analysis of high dimensional cytometry data

F Mair, FJ Hartmann, D Mrdjen… - European journal of …, 2016 - Wiley Online Library
Ever since its invention half a century ago, flow cytometry has been a major tool for single‐
cell analysis, fueling advances in our understanding of a variety of complex cellular systems …

Overview of clinical flow cytometry data analysis: recent advances and future challenges

CE Pedreira, ES Costa, Q Lecrevisse… - Trends in …, 2013 - cell.com
Major technological advances in flow cytometry (FC), both for instrumentation and reagents,
have emerged over the past few decades. These advances facilitate simultaneous …

Data analysis in flow cytometry: the future just started

E Lugli, M Roederer, A Cossarizza - Cytometry Part A, 2010 - Wiley Online Library
In the last 10 years, a tremendous progress characterized flow cytometry in its different
aspects. In particular, major advances have been conducted regarding the hardware …

A chromatic explosion: the development and future of multiparameter flow cytometry

PK Chattopadhyay, CM Hogerkorp, M Roederer - Immunology, 2008 - Wiley Online Library
Multiparameter flow cytometry has matured tremendously since the 1990s, giving rise to a
technology that allows us to study the immune system in unprecedented detail. In this article …

Statistical mixture modeling for cell subtype identification in flow cytometry

C Chan, F Feng, J Ottinger, D Foster… - Cytometry Part A …, 2008 - Wiley Online Library
Statistical mixture modeling provides an opportunity for automated identification and
resolution of cell subtypes in flow cytometric data. The configuration of cells as represented …

Cytobank: providing an analytics platform for community cytometry data analysis and collaboration

TJ Chen, N Kotecha - High-dimensional single cell analysis: Mass …, 2014 - Springer
Cytometry is used extensively in clinical and laboratory settings to diagnose and track cell
subsets in blood and tissue. High-throughput, single-cell approaches leveraging cytometry …

Mixture modeling approach to flow cytometry data

MJ Boedigheimer, J Ferbas - Cytometry Part A: The Journal of …, 2008 - Wiley Online Library
Flow Cytometry has become a mainstay technique for measuring fluorescent and physical
attributes of single cells in a suspended mixture. These data are reduced during analysis …