Feedback allows biological systems to control gene expression precisely and reliably, even
in the presence of uncertainty, by sensing and processing environmental changes. Taking …

RNA transcriptional regulators are emerging as versatile components for genetic network
construction. However, these regulators suffer from incomplete repression in their OFF state …

Highlights•Unique properties of RNA lead to the development of RNA-based dynamic
genetic controllers.•Natural riboswitches are re-engineered to detect new molecules.•RNA …

Our ability to program how cells express their genetic material is central to our ability to
understand and engineer them. While a range of biomolecules can be used to control gene …

Advances in synthetic biology have resulted in the development of genetic tools that support
the design of complex biological systems encoding desired functions. The majority of efforts …

Highlights•RNA offers highly compact, modular, portable, and programmable gene
regulation.•Ligand-responsive riboswitches allow for user-guided control of …

For synthetic biology applications, protein-based transcriptional genetic controllers are
limited in terms of orthogonality, modularity, and portability. Although ribozyme-based …

Highlights•We review recent progress in RNA synthetic biology.•New advances in synthetic
RNA regulator design have created high-performing/functioning parts.•New generation of …

RNA-based regulators are promising tools for building synthetic biological systems that
provide a powerful platform for achieving a complex regulation of transcription and …

Highlights•Feedback and feedforward motifs shape the steady state, temporal properties,
and noise profiles of gene expression systems.•Feedback and feedforward controllers make …