Oral delivery of dsRNA lipoplexes to German cockroach protects dsRNA from degradation and induces RNAi response

YH Lin, JH Huang, Y Liu, X Belles… - Pest management …, 2017 - Wiley Online Library
BACKGROUND In the past years, the concept of RNAi application for insect pest control has
been proposed, considering the disruption of vital genes. However, the efficiency of RNAi is …

Practical use of RNA interference: oral delivery of double-stranded RNA in liposome carriers for cockroaches

JH Huang, Y Liu, YH Lin, X Belles, HJ Lee - JoVE (Journal of Visualized …, 2018 - jove.com
RNA interference (RNAi) has been widely applied for uncovering the biological functions of
numerous genes, and has been envisaged as a pest control tool operating by disruption of …

Liposome encapsulation and EDTA formulation of dsRNA targeting essential genes increase oral RNAi‐caused mortality in the Neotropical stink bug Euschistus heros

NL Castellanos, G Smagghe, R Sharma… - Pest Management …, 2019 - Wiley Online Library
BACKGROUND The Neotropical stink bug Euschistus heros is a major pest in soybean
fields. Development of highly species‐specific pesticides based on RNA interference (RNAi) …

Ingested double-stranded RNAs can act as species-specific insecticides

S Whyard, AD Singh, S Wong - Insect biochemistry and molecular biology, 2009 - Elsevier
A serious shortcoming of many insecticides is that they can kill non-target species. To
address this issue, we harnessed the sequence specificity of RNA interference (RNAi) to …

[HTML][HTML] Insecticidal effects of dsRNA targeting the Diap1 gene in dipteran pests

M Powell, P Pyati, M Cao, H Bell, JA Gatehouse… - Scientific Reports, 2017 - nature.com
The Drosophila melanogaster (fruit fly) gene Diap1 encodes a protein referred to as DIAP1
(D rosophila I nhibitor of A poptosis P rotein 1) that acts to supress apoptosis in “normal” …

Delivery of dsRNA for RNAi in insects: an overview and future directions

N Yu, O Christiaens, J Liu, J Niu, K Cappelle… - Insect …, 2013 - Wiley Online Library
RNA interference (RNAi) refers to the process of exogenous double‐stranded RNA (dsRNA)
silencing the complementary endogenous messenger RNA. RNAi has been widely used in …

Variation in RNAi efficacy among insect species is attributable to dsRNA degradation in vivo

K Wang, Y Peng, J Pu, W Fu, J Wang, Z Han - Insect biochemistry and …, 2016 - Elsevier
RNA interference (RNAi) has become an essential technique in entomology research.
However, RNAi efficiency appears to vary significantly among insect species. Here, the …

Delivery of lethal dsRNAs in insect diets by branched amphiphilic peptide capsules

LA Avila, R Chandrasekar, KE Wilkinson… - Journal of controlled …, 2018 - Elsevier
Abstract Development of new and specific insect pest management methods is critical for
overcoming pesticide resistance and collateral off-target killings. Gene silencing by feeding …

Lipids help double‐stranded RNA in endosomal escape and improve RNA interference in the fall armyworm, Spodoptera frugiperda

D Gurusamy, K Mogilicherla… - Archives of Insect …, 2020 - Wiley Online Library
RNA interference (RNAi) is a valuable method for understanding the gene function and
holds great potential for insect pest management. While RNAi is efficient and systemic in …

A polymer/detergent formulation improves dsRNA penetration through the body wall and RNAi‐induced mortality in the soybean aphid Aphis glycines

Y Zheng, Y Hu, S Yan, H Zhou, D Song… - Pest Management …, 2019 - Wiley Online Library
BACKGROUND It is difficult to efficiently silence gene expression in some insects, probably
because of the degradation of dsRNA by enzymes present in the gut and hemolymph post …