Optimisation of sample storage and DNA extraction for human gut microbiota studies​

J Kazantseva, E Malv, A Kaleda, A Kallastu, A Meikas - BMC microbiology, 2021 - Springer
Background New developments in next-generation sequencing technologies and massive
data received from this approach open wide prospects for personalised medicine and …

Methodology challenges in studying human gut microbiota–effects of collection, storage, DNA extraction and next generation sequencing technologies

M Panek, H Čipčić Paljetak, A Barešić, M Perić… - Scientific reports, 2018 - nature.com
The information on microbiota composition in the human gastrointestinal tract predominantly
originates from the analyses of human faeces by application of next generation sequencing …

Combined bacterial and fungal intestinal microbiota analyses: Impact of storage conditions and DNA extraction protocols

C Angebault, A Ghozlane, S Volant, F Botterel… - PloS one, 2018 - journals.plos.org
Background The human intestinal microbiota contains a vast community of microorganisms
increasingly studied using high-throughput DNA sequencing. Standardized protocols for …

Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differences

B Wagner Mackenzie, DW Waite… - Frontiers in microbiology, 2015 - frontiersin.org
The human gut contains dense and diverse microbial communities which have profound
influences on human health. Gaining meaningful insights into these communities requires …

The effect of storage conditions on microbial communities in stool

K Nel Van Zyl, AC Whitelaw, M Newton-Foot - PLoS One, 2020 - journals.plos.org
Microbiome research has experienced a surge of interest in recent years due to the
advances and reduced cost of next-generation sequencing technology. The production of …

Fecal microbiota analysis: an overview of sample collection methods and sequencing strategies

V Thomas, J Clark, J Doré - Future microbiology, 2015 - Taylor & Francis
Despite huge interest, there are still no universally accepted standards to conduct clinical
studies in the field of gut microbiota analysis. Stool material is frequently used as a proxy of …

[HTML][HTML] Gut microbiota analysis results are highly dependent on the 16S rRNA gene target region, whereas the impact of DNA extraction is minor

A Rintala, S Pietilä, E Munukka, E Eerola… - Journal of …, 2017 - ncbi.nlm.nih.gov
Next-generation sequencing (NGS) is currently the method of choice for analyzing gut
microbiota composition. As gut microbiota composition is a potential future target for clinical …

[HTML][HTML] Exploring the universal healthy human gut microbiota around the World

S Piquer-Esteban, S Ruiz-Ruiz, V Arnau, W Diaz… - Computational and …, 2022 - Elsevier
The human gut holds a special place in the study of different microbial environments due to
growing evidence that the gut microbiota is related to host health. However, despite …

[HTML][HTML] Microbial culturomics: paradigm shift in the human gut microbiome study

JC Lagier, F Armougom, M Million, P Hugon… - Clinical Microbiology …, 2012 - Elsevier
Comprehensive determination of the microbial composition of the gut microbiota and the
relationships with health and disease are major challenges in the 21st century …

The effect of DNA extraction methodology on gut microbiota research applications

K Gerasimidis, M Bertz, C Quince, K Brunner… - BMC research …, 2016 - Springer
Background The effect that traditional and modern DNA extraction methods have on
applications to study the role of gut microbiota in health and disease is a topic of current …