Since its invention 29 years ago, two‐photon laser‐scanning microscopy has evolved from a
promising imaging technique, to an established widely available imaging modality used …

In vivo microscopy is a powerful method for studying fundamental issues of physiology and
pathophysiology. The recent development of multiphoton fluorescence microscopy has …

Intravital multiphoton microscopy is widely used to assess the structure and function of
organs in live animals. Although different tissues vary in their accessibility for intravital …

Multiphoton microscopy is based on the simultaneous absorption of two photons emitted by
a pulsed infrared laser source. In this technique, the excitation is restricted to a very small …

Over the past decade, two-photon microscopy has successfully made the transition from the
laser laboratory into a true biological research setting. This has been due in part to the …

Leaps in scientific technology often occur at the interface of seemingly disparate disciplines.
This holds true with the recent application of multiphoton microscopy to the biological …

In vivo imaging using two-photon microscopy is an essential tool to explore the dynamic of
physiological events deep within biological tissues for short or extended periods of time. The …

Serial intravital 2-photon microscopy of the kidney and other abdominal organs is a powerful
technique to assess tissue function and structure simultaneously and over time. Thus, serial …

Two‐photon excitation fluorescence microscopy and backscattered‐second harmonic
generation microscopy permit the investigation of the subcellular events within living …

Multi‐photon microscopy, now in its twentieth year, has developed into one of the most
robust and powerful techniques for live cell and in vivo fluorescence imaging. Although its …