Culture-independent methods have granted the possibility to study microbial diversity in
great detail, but technical issues pose a threat to the accuracy of new findings. Biases …

Introduction Determining bacterial community structure in fecal samples through DNA
sequencing is an important facet of intestinal health research. The impact of different …

To optimise fecal sampling for reproducible analysis of the gut microbiome, we compared
different methods of sample collection and sequencing of 16S rRNA genes at two centers …

Variability in representation of microbial communities can be caused by differences in
microbial composition or artifacts introduced at sample collection or processing. Alterations …

The human gut contains dense and diverse microbial communities which have profound
influences on human health. Gaining meaningful insights into these communities requires …

Several different protocols are used for fecal DNA extraction, which is an integral step in all
phylogenetic and metagenomic approaches to characterize the highly diverse intestinal …

The human gut harbors a vast range of microbes that have significant impact on health and
disease. Therefore, gut microbiome profiling holds promise for use in early diagnosis and …

Technical variation in metagenomic analysis must be minimized to confidently assess the
contributions of microbiota to human health. Here we tested 21 representative DNA …

Background DNA extraction is an important factor influencing the microbiome profile in fecal
samples. Considering that the QIAamp DNA Stool Mini Kit, one of the most commonly used …

Our understanding of human gut microbiota in health and disease depends on accurate and
reproducible microbial data acquisition. The critical step in this process is to apply an …