Sperm-mediated gene transfer (SMGT) is a method for the production of transgenic animals
based on the intrinsic ability of sperm cells to bind and internalize exogenous DNA …

Since 1989, a new method for the production of transgenic animals has been available,
namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to …

Transgenic animals are produced primarily by microinjecting exogenous DNA into the male
pronuclei of a zygote. Microinjection is successful in mice but not efficient in farm animals …

“Sperm mediated gene transfer”(SMGT) is based on the ability of sperm cells to bind
exogenous DNA. The main objective of this study was to improve the production of …

An efficient and low‐cost production of transgenic pigs has significant applications to the pig
industry and biomedical science. Generation of transgenic pig by sperm‐mediated gene …

In relation to the growing recent interest in the establishment of sperm‐mediated gene
transfer (SMGT) technology as a convenient and effective method for the simple production …

The pig reproduction section of this review covers semen sorting and preservation, in vitro
production of embryos, embryo splitting and nuclear transplantation, the production of …

Transgenic bovine sperm were produced by restriction enzyme mediated insertion (REMI).
REMI utilizes lipofection of linearized pEGFP and the corresponding restriction enzyme for …

Understanding the behavior of transgenes introduced into oocytes or embryos is essential
for evaluating the methodologies for transgenic animal production. We investigated the …

A simple and efficient method for producing multitransgenic animals is required for medical
and veterinary applications. Sperm-mediated gene transfer (SMGT) is an effective method …