Synthetic biology integrates diverse engineering disciplines to create novel biological
systems for biomedical and technological applications. The substantial growth of the …

Cell-free gene expression systems present an alternative approach to synthetic biology,
where biological gene expression is harnessed inside non-living, in vitro biochemical …

Cell-free synthetic biology offers an unprecedented level of control and freedom of design
for understanding, harnessing, and expanding the capabilities of natural biological systems …

Cell-free transcription–translation is an expanding field in synthetic biology as a rapid
prototyping platform for blueprinting the design of synthetic biological devices. Exemplar …

The practitioners of the brand of synthetic biology that lies closest to engineering are fond of
quoting the phrase Richard Feynman had written on his blackboard:'What I cannot create I …

Synthetic biology is focused on the rational construction of biological systems based on
engineering principles. During the field's first decade of development, significant progress …

The advent of synthetic biology has ushered in new applications of cell-free transcription-
translation systems. These cell-free systems are reconstituted using cellular proteins, and …

In parallel with the development of high-throughput technologies fueling systems biology,
advances in modeling of biological systems and in synthesis of long DNA fragments with …

Highlights•Cell-free expression is a highly multidisciplinary bioengineering technology.•Cell-
free expression is fast, quantitative and a safe synthetic biology environment.•TXTL …

Synthetic biology is the discipline of engineering application-driven biological functionalities
that were not evolved by nature. Early breakthroughs of cell engineering, which were based …