A Gallo, N Cuscino, F Contino, M Bulati… - International Journal of …, 2022 - mdpi.com
Mesenchymal stromal/stem cells (MSCs) are believed to function in vivo as a homeostatic tool that shows therapeutic properties for tissue repair/regeneration. Conventionally, these …
While traditional cell culture methods have relied on growing cells as monolayers, three- dimensional (3D) culture systems can provide a convenient in vitro model for the study of …
W Wang, K Itaka, S Ohba, N Nishiyama, U Chung… - Biomaterials, 2009 - Elsevier
Multipotent mesenchymal stem cells (MSCs) are one of the most powerful tools in regeneration medicine. Their low differentiation efficiency, however, limits further application …
Y Zhou, H Chen, H Li, Y Wu - Journal of cellular and molecular …, 2017 - Wiley Online Library
Abstract Three‐dimensional (3D) culture has been shown to improve pluripotent gene expression in mesenchymal stem cells (MSC s), but the underlining mechanisms were …
A Kaminska, A Wedzinska, M Kot, A Sarnowska - Cells, 2021 - mdpi.com
The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural …
HY Yeh, BH Liu, M Sieber, S Hsu - BMC genomics, 2014 - Springer
Abstract Background Three-dimensional (3D) multicellular spheroids of mesenchymal stem cells (MSCs) are generally regarded to have beneficial properties over MSCs in monolayer …
A Imamura, H Kajiya, S Fujisaki, M Maeshiba… - Biochemical and …, 2020 - Elsevier
Mesenchymal stem/stromal cells (MSCs) are multipotent and self-renewal cells that are widely used in regenerative medicine. The culture of three-dimensional (3D) spheroid MSCs …
Z Cesarz, K Tamama - Stem cells international, 2016 - Wiley Online Library
Compared with traditional 2D adherent cell culture, 3D spheroidal cell aggregates, or spheroids, are regarded as more physiological, and this technique has been exploited in the …
L Yan, B Jiang, E Li, X Wang, Q Ling… - … journal of biological …, 2018 - ncbi.nlm.nih.gov
Human embryonic stem cell (hESC) derived mesenchymal stem cells (EMSC) are efficacious in treating a series of autoimmune, inflammatory, and degenerative diseases in …