PCR for detection of herpes simplex virus in cerebrospinal fluid: alternative acceptance criteria for diagnostic workup

P Lopez Roa, R Alonso, V de Egea… - Journal of Clinical …, 2013 - Am Soc Microbiol
The determination of herpes simplex virus (HSV) infection using a PCR assay is one of the
most commonly requested tests for analysis of cerebrospinal fluid (CSF), although only a …

Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid

V Hukkanen, T Rehn, R Kajander… - Journal of clinical …, 2000 - Am Soc Microbiol
We have introduced a time-resolved fluorometry (TRF)-based microwell hybridization assay
for PCR products in detection of herpes simplex virus (HSV) in cerebrospinal fluid (CSF) …

Validation of laboratory screening criteria for herpes simplex virus testing of cerebrospinal fluid

KE Hanson, BD Alexander, C Woods… - Journal of Clinical …, 2007 - Am Soc Microbiol
Most patients with herpes simplex virus (HSV) central nervous system (CNS) infection have
abnormal cerebrospinal fluid (CSF) indices. Therefore, we implemented screening criteria …

Appropriate use of polymerase chain reaction for detection of herpes simplex virus 2 in cerebrospinal fluid of patients at an inner-city hospital

N Afonso, S Gunasena, K Galla, R Podzorski… - … and infectious disease, 2007 - Elsevier
Polymerase chain reaction (PCR) tests that detect herpes simplex virus (HSV) DNA in
cerebrospinal fluid (CSF) are increasingly used to diagnose central nervous system (CNS) …

Rapid and direct detection of herpes simplex virus in cerebrospinal fluid by use of a commercial real-time PCR assay

MJ Binnicker, MJ Espy, CL Irish - Journal of Clinical Microbiology, 2014 - Am Soc Microbiol
Central nervous system infection due to herpes simplex virus (HSV) is a medical emergency
and requires rapid diagnosis and initiation of therapy. In this study, we compared a routine …

Laboratory diagnosis of central nervous system infections with herpes simplex virus by PCR performed with cerebrospinal fluid specimens

PS Mitchell, MJ Espy, TF Smith, DR Toal… - Journal of clinical …, 1997 - Am Soc Microbiol
Until recently, the laboratory diagnosis of central nervous system (CNS) infections with
herpes simplex virus (HSV) has been limited by poor sensitivity and/or specificity. We …

Detection of herpes simplex virus DNA from cerebrospinal fluid by PCR and a rapid, nonradioactive hybridization technique

HH Kessler, K Pierer, B Weber… - Journal of clinical …, 1994 - Am Soc Microbiol
A molecular assay for the detection of herpes simplex virus (HSV), including a novel,
nonradioactive hybridization technique, was evaluated with a total of 123 cerebrospinal fluid …

Two‐centre study comparing DNA preparation and PCR amplification protocols for herpes simplex virus detection in cerebrospinal fluids of patients with suspected …

HH Hirsch, W Bossart - Journal of medical virology, 1999 - Wiley Online Library
In a two‐centre study, the routine DNA preparation and PCR amplification protocols were
compared for herpes simplex virus (HSV) detection in cerebrospinal fluids (CSFs) of 43 …

Evaluation of three analyte-specific reagents for detection and typing of herpes simplex virus in cerebrospinal fluid

SB Selvaraju, M Wurst, RT Horvat… - … and infectious disease, 2009 - Elsevier
The performance of 3 analyte-specific reagents (ASRs), viz Cepheid herpes simplex virus
(HSV) Typing Primer Probe set (CD), Eragen MultiCode-Rtx HSV-1/2 kit primer mix (ER) …

Clinical spectrum and laboratory characteristics associated with detection of herpes simplex virus DNA in cerebrospinal fluid

CE O'Sullivan, AJ Aksamit, JR Harrington… - Mayo Clinic …, 2003 - Elsevier
Objective To determine the clinical, neurologic, and laboratory characteristics of patients
with herpes simplex virus (HSV) type 1 (HSV-1) or HSV type 2 (HSV-2) DNA detected in …