Repressor forms of the enhancer-binding protein NtrC: some fail in coupling ATP hydrolysis to open complex formation by σ54-holoenzyme

AK North, DS Weiss, H Suzuki, Y Flashner… - Journal of molecular …, 1996 - Elsevier
NtrC (nitrogen regulatory protein C) is a bacterial enhancer-binding protein that activates
transcription by catalyzing isomerization of closed complexes between σ54-holoenzyme and …

Constitutive forms of the enhancer-binding protein NtrC: evidence that essential oligomerization determinants lie in the central activation domain

Y Flashner, DS Weiss, J Keener, S Kustu - Journal of molecular biology, 1995 - Elsevier
Nitrogen regulatory protein C (NtrC) is a bacterial enhancer-binding protein that activates
transcription by the σ54-holoenzyme. To activate transcription, NtrC must hydrolyze ATP, a …

“Switch I” mutant forms of the bacterial enhancer-binding protein NtrC that perturb the response to DNA

D Yan, S Kustu - Proceedings of the National Academy of …, 1999 - National Acad Sciences
NtrC (nitrogen regulatory protein C) is a bacterial enhancer-binding protein of 469 residues
that activates transcription by σ54-holoenzyme. A region of its transcriptional activation …

Mechanism of transcriptional activation by NtrC

SC Porter, AK North, S Kustu - Two‐Component Signal …, 1995 - Wiley Online Library
In the case of nitrogen regulatory protein C (NtrC), both nucleotide hydrolysis and
transcriptional activation depend on phosphorylation of an aspartate residue in the N …

Physical evidence for a phosphorylation-dependent conformational change in the enhancer-binding protein NtrC

I Hwang, T Thorgeirsson, J Lee… - Proceedings of the …, 1999 - National Acad Sciences
The bacterial enhancer-binding protein nitrogen regulatory protein C (NtrC) activates
transcription by σ54-containing RNA polymerase in a reaction that depends on ATP …

The major dimerization determinants of the nitrogen regulatory protein NTRC from enteric bacteria lie in its carboxy-terminal domain

KE Klose, AK North, KM Stedman, S Kustu - Journal of molecular biology, 1994 - Elsevier
The NTRC protein (nitrogen regulatory protein C) of enteric bacteria is an enhancer-binding
protein that activates transcription by the σ 54-holoenzyme form of RNA polymerase. NTRC …

Mutant forms of the enhancer-binding protein NtrC can activate transcription from solution

AK North, S Kustu - Journal of molecular biology, 1997 - Elsevier
Activators of the σ54-holoenzyme catalyze the isomerization of closed complexes between
this polymerase and a promotor to open complexes in a reaction that depends upon …

Glutamate at the site of phosphorylation of nitrogen-regulatory protein NTRC mimics aspartyl-phosphate and activates the protein

KE Klose, DS Weiss, S Kustu - Journal of molecular biology, 1993 - Elsevier
The NTRC protein of enteric bacteria is an enhancer-binding protein that activates
transcription by the σ 54-holoenzyme form of RNA polymerase under nitrogen-limiting …

The prokaryotic enhancer binding protein NTRC has an ATPase activity which is phosphorylation and DNA dependent.

S Austin, R Dixon - The EMBO journal, 1992 - embopress.org
The prokaryotic activator protein NTRC binds to enhancer‐like elements and activates
transcription in response to nitrogen limitation by catalysing open complex formation by …

Mutations affecting motifs of unknown function in the central domain of nitrogen regulatory protein C

J Li, L Passaglia, I Rombel, D Yan… - Journal of bacteriology, 1999 - Am Soc Microbiol
The positive control function of the bacterial enhancer-binding protein NtrC resides in its
central domain, which is highly conserved among activators of ς54 holoenzyme. Previous …