The detection limits of fluorescein and riboflavin are determined to be 0.02 and 0.6 parts-per-
trillion, respectively, with the use of a very sensitive fluorometric system (a nitrogen …

Sir: Fluorometric analysis provides a useful method for the determination of the ppb level of
the fluorescing molecule. In principle, the sensitivity can be raised by using a more intense …

Sir: It is well-known that laser-induced fluorescence is a very sensitive technique for
ultratrace analysis. Various combinations of laser sources and detection systems have been …

A new type of spectrofluorometer has been developed which utilizes a single modified
monochromator both as a tunable dye laser excitation source and as a scanning mo …

Conventional fluorescence finds many applications in analytical chemistry because of its
sensitivity and selectivity f I] _ The recent use of lasers instead of conventional light sources …

We wish to summarise some of the factors which must be considered in order to choose the
best possible conditions for fluorimetric determinations: 1. The absorption spectrum of the …

The effect of excitation beam absorption on measured values of fluorescence has been
studied with a computer-centered spectrofluorlmeter capable of measuring fluorescence and …

A fluorlmeter employing single photon counting and mode-locked laser excitation was
constructed to test the concept of time-filtered detection. Expressions for a figure of merit and …

Most spectrofluorometers re-cord “apparent” excitation and emission spectra which are a
function of the particular instrument used. Such apparent spectra are often grossly distorted …

Table I. Characteristics of Organic Dyes in theDeep-Red Region molar absorption
absorptivity, fluorescence saturated compound max, nm(M cm)" 1 max, nm concn, M …