Application of phospholipid fatty acid method in soil microbial analysis

S Wang, Y Hou - Microbiology, 1992 - pesquisa.bvsalud.org
Phospholipid fatty acids are the major constituents of the membranes of all living cells, and
different groups of microorganism synthesize a variety of PLFA through various biochemical …

High turnover rate of free phospholipids in soil confirms the classic hypothesis of PLFA methodology

Y Zhang, N Zheng, J Wang, H Yao, Q Qiu… - Soil Biology and …, 2019 - Elsevier
Phospholipid fatty acid (PLFA) analysis has been widely used to study soil microbial
community structure. Soil PLFAs are taken to be indicative of living organisms since …

Phospholipid fatty acid analysis and its applications in microbial ecology

Q Hongyan, X Kai, Z Hongxun - Acta Ecologica Sinica, 2003 - europepmc.org
The quantitative description of microbial community is one of the difficult tasks for microbial
ecologist. Conventional cultivation techniques cannot be used to fully characterize most soil …

The use of phospholipid fatty acid analysis to estimate bacterial and fungal biomass in soil

A Frostegård, E Bååth - Biology and Fertility of soils, 1996 - Springer
The cell content of 12 bacterial phospholipid fatty acids (PLFA) was determined in bacteria
extracted from soil by homogenization/centrifugation. The bacteria were enumerated using …

[PDF][PDF] 8.3 Phospholipid Fatty Acid (PLFA) Analyse s

A Palojärvi - METHODS FOR ASSESSING SOIL QUALITY, 2006 - fsp-parrur.irenala.edu.mg
The analysis of ester-linked (EL) phospholipid fatty acids (PLFAs) is an acknowledged
biochemical approach to microbial community characterization. PLFAs are constituents of all …

Use and misuse of PLFA measurements in soils

Å Frostegård, A Tunlid, E Bååth - Soil Biology and Biochemistry, 2011 - Elsevier
The determination of the phospholipid fatty acid (PLFA) pattern of soil organisms has
become one of the most commonly used methods to study microbial community structure …

[PDF][PDF] Phospholipid fatty acid (PLFA) analysis: a robust indicator for soil health?

WF Brinton - Soil Biology & Biochemistry, 2020 - researchgate.net
Opinion Phospholipid fatty acid, commonly referred to as PLFA, is an analytical concept that
emerged in the late 1980's for estimating composition of soil microbial communities [1] …

[HTML][HTML] High throughput phospholipid fatty acid analysis of soils

JS Buyer, M Sasser - Applied Soil Ecology, 2012 - Elsevier
Phospholipid fatty acid (PLFA) analysis is widely used to measure microbial biomass and
community composition in soil and other types of environmental samples. As typically …

Extraction parameters significantly influence the quantity and the profile of PLFAs extracted from soils

ES Papadopoulou, DG Karpouzas… - Microbial ecology, 2011 - Springer
Phospholipid fatty acid (PLFA) analysis is a robust method for characterizing soil microbial
communities. We determined the effects of extraction solvent (chloroform vs …

Application of phospholipid fatty acid (PLFA) analysis in microbial ecology

Z CHEN, X YU, M XIA, SUN DAI Zhaoxia - Chinese Journal of Ecology, 2005 - cje.net.cn
Phospholipid fatty acid (PLFA) analysis, based on modern biochemical techniques, is widely
used in the research of microbial ecology nowadays. It shows many advantages over the …