Expression optimization and synthetic gene networks in cell-free systems

DK Karig, S Iyer, ML Simpson… - Nucleic acids …, 2012 - academic.oup.com
Synthetic biology offers great promise to a variety of applications through the forward
engineering of biological function. Most efforts in this field have focused on employing living …

[HTML][HTML] Cell-free synthetic biology platform for engineering synthetic biological circuits and systems

D Jeong, M Klocke, S Agarwal, J Kim, S Choi… - Methods and …, 2019 - mdpi.com
Synthetic biology integrates diverse engineering disciplines to create novel biological
systems for biomedical and technological applications. The substantial growth of the …

Cell-free gene expression: an expanded repertoire of applications

AD Silverman, AS Karim, MC Jewett - Nature Reviews Genetics, 2020 - nature.com
Cell-free biology is the activation of biological processes without the use of intact living cells.
It has been used for more than 50 years across the life sciences as a foundational research …

Host-aware synthetic biology

A Boo, T Ellis, GB Stan - Current Opinion in Systems Biology, 2019 - Elsevier
Unnatural gene expression imposes a load on engineered microorganisms which
decreases their growth and subsequent production yields, a phenomenon known as burden …

[HTML][HTML] Transferring a synthetic gene circuit from yeast to mammalian cells

D Nevozhay, T Zal, G Balázsi - Nature communications, 2013 - nature.com
The emerging field of synthetic biology builds gene circuits for scientific, industrial and
therapeutic needs. Adaptability of synthetic gene circuits across different organisms could …

An E. coli Cell-Free Expression Toolbox: Application to Synthetic Gene Circuits and Artificial Cells

J Shin, V Noireaux - ACS synthetic biology, 2012 - ACS Publications
Cell-free protein synthesis is becoming a powerful technique to construct and to study
complex informational processes in vitro. Engineering synthetic gene circuits in a test tube …

Multiplex transcriptional characterizations across diverse bacterial species using cell‐free systems

SS Yim, NI Johns, J Park, ALC Gomes… - Molecular Systems …, 2019 - embopress.org
Cell‐free expression systems enable rapid prototyping of genetic programs in vitro.
However, current throughput of cell‐free measurements is limited by the use of channel …

Decoupling resource-coupled gene expression in living cells

T Shopera, L He, T Oyetunde, YJ Tang… - ACS synthetic …, 2017 - ACS Publications
Synthetic biology aspires to develop frameworks that enable the construction of complex
and reliable gene networks with predictable functionalities. A key limitation is that increasing …

[HTML][HTML] Systems biology strikes gold

DM Camacho, JJ Collins - Cell, 2009 - cell.com
Integrating synthetic biology and systems biology efforts can advance our understanding of
biomolecular systems. This is illustrated in this issue by Cantone et al.(2009), who construct …

[HTML][HTML] Transcription control engineering and applications in synthetic biology

MD Engstrom, BF Pfleger - Synthetic and systems biotechnology, 2017 - Elsevier
In synthetic biology, researchers assemble biological components in new ways to produce
systems with practical applications. One of these practical applications is control of the flow …