Optimization of spermatozoa analysis in mice: a comprehensive protocol

CP Vieira, MS Procópio, GF de Avelar - Tissue and Cell, 2024 - Elsevier
Sperm quality is critical to predict reproductive alterations caused by immunological factors
or toxicant agents. Yet, no detailed protocol has been published focusing on analyses of …

Cut-off values for normal sperm morphology and toxicology for automated analysis of rat sperm morphology and morphometry

G van Der Horst, B Skosana, A Legendre… - Biotechnic & …, 2018 - Taylor & Francis
We used automated sperm morphology analysis to investigate rat sperm morphometry and
morphology in Sprague-Dawley and Wistar rats in three research centers to develop normal …

First-line evaluation of sperm parameters in mice (Mus musculus)

G Martinez - Bio-protocol, 2022 - bio-protocol.org
Infertility has become a major public health problem, with a male factor involved in about half
the cases. Mice are the most widely used animal model in reproductive biology research …

The relationship between sperm morphology and in vitro fertilization ability in mice

Y Kawai, T Hata, O Suzuki, J Matsuda - Journal of Reproduction and …, 2006 - jstage.jst.go.jp
In vitro fertilization (IVF) is widely used in reproduction research, but the sperm of some
inbred strains of mice yield low fertilization rates in IVF. To determine the cause of this …

Mouse spermatozoa with higher fertilization rates have thinner nuclei

D Mashiko, M Ikawa, K Fujimoto - PeerJ, 2017 - peerj.com
Background Although spermatozoa with normal morphology are assumed to have uniform
fertilization ability, recent data show that even normal spermatozoa have considerable …

Multiple flow cytometry analysis for assessing human sperm functional characteristics

J de Lima Rosa, CPF Dell'Aqua, FF de Souza… - Reproductive …, 2023 - Elsevier
Multiparametric analysis by flow cytometry solves one of the major problems in sperm
evaluation, the inability to test multiple attributes simultaneously in a single cell, which would …

Sperm morphology is different in two common mouse strains

N Sekine, S Yokota, S Oshio - BPB Reports, 2021 - jstage.jst.go.jp
Abstract ICR and C57BL/6J mice have been widely used in several research fields. The
reproductive toxicology parameters, such as fertilization rate, which may differ between the …

[HTML][HTML] Histology, spermatogenesis

SH Suede, A Malik, A Sapra - 2020 - europepmc.org
The union of male and female gametes creates offspring. The production of these vital
reproductive cells occurs in the testis and ovary during the processes of spermatogenesis …

Measuring mouse sperm parameters using a particle counter and sperm quality analyzer: a simple and inexpensive method

K Tayama, H Fujita, H Takahashi, A Nagasawa… - Reproductive …, 2006 - Elsevier
This study examined a method for analyzing the count, motility, and morphology of mouse
epididymal sperm, optimizing the diluent, incubation time, sample concentration, and …

Assessments of sperm quality integrating morphology, swimming patterns, bioenergetics and cell signalling

ERS Roldan - Theriogenology, 2020 - Elsevier
Spermatozoa are diverse in form and function and these differences impact on their
fertilizing capacity. Because of considerable inter-male and inter-species differences in …