Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable
measure for taxonomic profiling of bacterial communities. This chapter describes the …

Amplicon sequencing of the 16S rRNA gene is commonly used for the identification of
bacterial isolates in diagnostic laboratories and mostly relies on the Sanger sequencing …

Rapid identification of bacterial pathogens is crucial for appropriate and adequate antibiotic
treatment, which significantly improves patient outcomes. 16S ribosomal RNA (rRNA) gene …

Due to recent improvements, Nanopore sequencing has become a promising method for
experiments relying on amplicon sequencing. We describe a flexible workflow to generate …

Background The study of microbial communities and their applications have been leveraged
by advances in sequencing techniques and bioinformatics tools. The Oxford Nanopore …

Background Accurate identification of bacterial communities is crucial for research
applications, diagnostics, and clinical interventions. Although 16S ribosomal RNA (rRNA) …

While 16S rRNA PCR-Sanger sequencing has paved the way for the diagnosis of culture-
negative bacterial infections, it does not provide the composition of polymicrobial infections …

This study describes the implementation of 16S nanopore sequencing in a diagnostic lab for
pathogen identification without prior enrichment. First, the universality of the test and …

The prolonged incubation period of traditional culture methods leads to a delay in
diagnosing invasive infections. Nanopore 16S rRNA gene sequencing (Nanopore 16S) …

Next-generation sequencing has revolutionized the field of microbiology research and
greatly expanded our knowledge of complex bacterial communities. Nanopore sequencing …