The protein-coding regions of most vertebrate genes are split, giving rise to primary
transcripts containing introns and exons; the latter must be spliced in the nucleus to form the …

A large number of genes express multiple mRNAs that encode diverse protein isoforms via
alternative pre-mRNA splicing. For many genes, alternative splicing is regulated according …

We set up an alternative splicing system in vitro in which the relative amounts of two spliced
RNAs, one containing and the other lacking a particular exon, were directly proportional to …

We have recently demonstrated that short internal exons in pre-mRNA transcripts with three
exons and two introns are ignored by splicing machinery in vitro and in vivo, resulting in …

We analyzed the in vitro splicing pathways of three multi-intervening-sequence (IVS) pre-
mRNAs: human β-globin, which contains two IVSs (KM Lang, VL van Santen, and RA Spritz …

A characteristic feature of eukaryotic cells is that the primary transcription products of their
structural genes (pre-mRNAs) undergo a variety of post transcriptional modifications to …

The primary transcripts of most nuclear protein-coding genes of eu-karyotes are interrupted
by intervening sequences (or introns) that are removed from the pre-mRNA in a process …

Splicing of nuclear precursor messenger RNAs is an important and ubiquitous type of gene
regulation in metazoans. Splicing joins the coding sequences called exons by removing the …

Biochemical components(splicing factors) interact with specific intron regions during pre-
mRNA splicing in vitro. The pm-mRNA specifically associates with factors at both the branch …

The in vitro splicing assay is a valuable technique that can be used to study the mechanism
and machinery involved in the splicing process. The ability to investigate various aspects of …