[HTML][HTML] Comparison of DNA extraction methods for human gut microbial community profiling

MY Lim, EJ Song, SH Kim, J Lee, YD Nam - Systematic and applied …, 2018 - Elsevier
The human gut harbors a vast range of microbes that have significant impact on health and
disease. Therefore, gut microbiome profiling holds promise for use in early diagnosis and …

[HTML][HTML] The impact of DNA extraction methods on stool bacterial and fungal microbiota community recovery

K Fiedorová, M Radvanský, E Němcová… - Frontiers in …, 2019 - frontiersin.org
Our understanding of human gut microbiota in health and disease depends on accurate and
reproducible microbial data acquisition. The critical step in this process is to apply an …

[HTML][HTML] Evaluating variation in human gut microbiota profiles due to DNA extraction method and inter-subject differences

B Wagner Mackenzie, DW Waite… - Frontiers in microbiology, 2015 - frontiersin.org
The human gut contains dense and diverse microbial communities which have profound
influences on human health. Gaining meaningful insights into these communities requires …

An improved methodology to overcome key issues in human fecal metagenomic DNA extraction

J Kumar, M Kumar, S Gupta, V Ahmed… - Genomics …, 2016 - academic.oup.com
Microbes are ubiquitously distributed in nature, and recent culture-independent studies have
highlighted the significance of gut microbiota in human health and disease. Fecal DNA is the …

[HTML][HTML] The impact of different DNA extraction kits and laboratories upon the assessment of human gut microbiota composition by 16S rRNA gene sequencing

NA Kennedy, AW Walker, SH Berry, SH Duncan… - PloS one, 2014 - journals.plos.org
Introduction Determining bacterial community structure in fecal samples through DNA
sequencing is an important facet of intestinal health research. The impact of different …

[HTML][HTML] A comparison of the efficiency of five different commercial DNA extraction kits for extraction of DNA from faecal samples

S Claassen, E du Toit, M Kaba, C Moodley… - Journal of …, 2013 - Elsevier
Differences in the composition of the gut microbiota have been associated with a range of
diseases using culture-independent methods. Reliable extraction of nucleic acid is a key …

[HTML][HTML] The effect of DNA extraction methodology on gut microbiota research applications

K Gerasimidis, M Bertz, C Quince, K Brunner… - BMC research …, 2016 - Springer
Background The effect that traditional and modern DNA extraction methods have on
applications to study the role of gut microbiota in health and disease is a topic of current …

Comparative analysis of fecal DNA extraction methods with phylogenetic microarray: effective recovery of bacterial and archaeal DNA using mechanical cell lysis

A Salonen, J Nikkilä, J Jalanka-Tuovinen… - Journal of …, 2010 - Elsevier
Several different protocols are used for fecal DNA extraction, which is an integral step in all
phylogenetic and metagenomic approaches to characterize the highly diverse intestinal …

[PDF][PDF] Assessment of fecal DNA extraction protocols for metagenomic studies

F Yang, J Sun, H Luo, H Ren, H Zhou, Y Lin… - …, 2020 - academic.oup.com
Background Shotgun metagenomic sequencing has improved our understanding of the
human gut microbiota. Various DNA extraction methods have been compared to find …

Progress of analytical tools and techniques for human gut microbiome research

EJ Song, ES Lee, YD Nam - Journal of Microbiology, 2018 - Springer
Massive DNA sequencing studies have expanded our insights and understanding of the
ecological and functional characteristics of the gut microbiome. Advanced sequencing …